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A 3-week Biotech Unit for Any Biology Class

A 3-week Biotech Unit for Any Biology Class. Ellyn Daugherty SMBCP, San Mateo CA Educator/Author www.BiotechEd.com www.SMBiotech.com www.emcp.com/biotech www.sargentwelch.com/biotech. Biotech in Biology 3 weeks (15 hours). > What is Biotechnology? Computer Search Collage

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A 3-week Biotech Unit for Any Biology Class

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  1. A 3-week Biotech Unitfor Any Biology Class Ellyn Daugherty SMBCP, San Mateo CA Educator/Author www.BiotechEd.com www.SMBiotech.com www.emcp.com/biotech www.sargentwelch.com/biotech

  2. Biotech in Biology3 weeks (15 hours) > What is Biotechnology? Computer Search Collage > Micropipeting Activity > DNA Spooling Activity > DNA Fingerprinting Using RFLPs Activity > Recombinant DNA Technology > Bacterial Transformation (GMO) Activity > Bioethics of Engineering Organisms > Careers in Biotechnology

  3. Materials, Supplies, Funds Needed for Biotech in Biology Unit • Class set of: • 4 micropipets/tips each (P-1000, P-200, P-20) • 4 gel boxes, 2 power supplies • DNA Spooling Activity Kit • DNA Fingerprinting Activity Kit • Bacteria Transformation Activity Kit • Other supplies and materials (96-well plates, food coloring, 37°C incubation oven, water bath = about $300 in kits/ class = about $3000 for school set of micropipets and gel apparatus

  4. What Is Biotechnology?

  5. Micropipeting Activity Show me: Pipet 135 µL or 235 µL into a tube

  6. DNA Spooling Activity What Does DNA Look Like? (Edvotek #S-10) = one 50 min class period (30 min activity, 20 min discussion $39 / 10 lab groups Do Onions Have DNA (or other fruits or veggies) ? (Edvotek #S-75) = one 50 min class period (30 min activity, 20 min discussion $49 / 5 lab groups … or use my Lab 4b Materials from SW List

  7. DNA Fingerprinting Using RFLPs Activity Ward’s DNA Detectives Lab Activity #109) = four 50 min class periods $115 / 8 lab groups Edvotek’s DNA Fingerprinting by Restriction Fragmentation Patterns #109) = four 50 min class periods $79 / 6 lab groups

  8. Recombinant DNA Technologyuse http://www.dnalc.org/resources/animations/ • Use the Restriction Digestion animation to discuss: • Restriction enzymes • DNA ligase • Recombinant DNA (rDNA) • Use the Transformation animation to discuss: • Insertion of rDNA vector • Selection and Expression of newly insert genes • Applications of rDNA technology

  9. Bacterial Transformation (GMO) Activity Ward’s Improved Bacterial Transformation Using GFP #36 V 6359 = four 50 min class periods $85/ 8 lab groups Edvotek’s Transformation with Green Fluorescent Protein #223 and #969 = four 50 min class periods $110/ 10 lab groups

  10. Biotechnology Career Research e-Project

  11. Bioethics of Engineered Organisms

  12. Materials Lists www.sargentwelch.com/biotech Find your Sales Rep for materials/equipment/ support

  13. Biotechnology: Science for the New Millennium Ellyn Daugherty ellyn@BiotechEd.com • Text with Encore CD • Lab Manual • Instructor’s Guide • Course Planner (Lesson Plans) • Instructor Resource Center on web • Student Notebook, Test Generator, etc. available www.BiotechEd.com for tour through the entire curriculum, ancillaries, websites, and professional development opportunities

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