1 / 10

Programmed population control by cell-cell communication and regulated killing

Programmed population control by cell-cell communication and regulated killing. Zhang Junqiu Paper presentation Nature 2004, 428:868-871. Introduction. We want to build a quorum-sensing system which can control cell density at a constant amount autonomously.

taffy
Download Presentation

Programmed population control by cell-cell communication and regulated killing

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Programmed population control by cell-cell communication and regulated killing Zhang Junqiu Paper presentation Nature 2004, 428:868-871

  2. Introduction • We want to build a quorum-sensing system which can control cell density at a constant amount autonomously. • Mechanism similar to natural circuit (Streptococcus pneumoniae肺炎链球菌)

  3. How to create such a cell? • Gene that can produce toxic protein • The gene that can produce something that can be a signal • The signaling molecule can be sensed by other cells • Then the killer gene can be activated by the signaling molecule • Reality can be very difficult!

  4. Explanation of some words • AHL: acyl-homoserinelactone（酰基高丝氨酸内酯）; • LuxI, LuxR, LacZα, ccdB: protein; • pluxI , plac/ara-1: promoters; • pLuxRI2, pluxCcdB3: plasmids; • LacZα-ccdB: killer gene;

  5. The circuit • LuxI→ AHL (accumulate)→ LuxR→ E • I: LuxI • R: LuxR • R*: active LuxR • E: killer gene • pluxI: promoter

  6. Implement the circuit • Two-plasmid system • Isopropyl-β-D-thiogalactopyranoside (IPTG)→ pLuxRI2; activated LuxR→ pluxCcdB3 • T0, T1: transcription terminators

  7. Mathematical modelling • Cell density: N; carrying capacity: Nm; concentration of killer protein: E; concentration of AHL: A; • Growth constant: k; killing constant: d; (production rate of E/A): kE; (AHL production rate/N):vA; degradation of AHL& killer protein:dA& dE.

  8. Experiment result

  9. Q & A

  10. Thank you !

More Related