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Apaf-1

control. treated. control. treated. E40 - +. E40 - +. E40 40 h. E50 24 h. IP : Apaf-1 WB: AChE. IP: c-IAP-1 WB: AChE. TE671. b -actin. b -actin. IP: c-IAP-2 WB: AChE. IP: Caspase-3 WB: AChE. Sulindac 1 mM, 48 h. E40 24 h. U373MG. b -actin. b -actin.

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Apaf-1

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  1. control treated control treated E40 - + E40 - + E40 40 h E50 24 h IP : Apaf-1 WB: AChE IP: c-IAP-1 WB: AChE TE671 b-actin b-actin IP: c-IAP-2 WB: AChE IP: Caspase-3 WB: AChE Sulindac 1 mM, 48 h E40 24 h U373MG b-actin b-actin IP: Caspase-9 WB: AChE IP: Cyt c WB: AChE HT-29 E10 48 h LY294002 100 M, 24 h SK-MEL5 b-actin b-actin Thapsigargin 2.5 M, 24 h E50 24 h Malme-3M b-actin b-actin SIN-1 1 mM, 24 h SNP 1.5 mM, 24 h RAW 264.7 b-actin b-actin Chondrocyte TRAIL 100 MOI, 72 h IL-3 deprivation BMMC b-actin b-actin HT-29 Chondrocyte RAW 264.7 thapsigargin LY294002 sulindac control control control control control TRAIL control SIN-1 E Apaf-1 Caspase-9 Cyt c IP: AChE Supplementary Fig. S1 A B C

  2. - - + - - + + + E40 si Cyt c Cyt c Hoechst si Cyt c PC 0 8 16 24 48 h IP: Apaf-1 WB: Apaf-1 Apaf-1 HT-29 Thapsigargin 2.5 M 105 IgG IgG 50 IP: Apaf-1 WB: Apaf-1 Apaf-1 Chondrocyte TRAIL 100 MOI 105 IgG IgG 50 IP: AChE si Cyt c 0 8 16 24 48 h PC IP: Apaf-1 WB: Apaf-1 Apaf-1 RAW 264.7 SIN-1 1 mM 105 IgG IgG 50 IP: Apaf-1 WB: Apaf-1 Apaf-1 105 SK-MEL-5 E10 IgG IgG 50 IP: AChE Supplementary Fig. S2 A B

  3. E40 0 8 16 24 48 72 h MbCD E40 - - - + 1/12 + 1 + 2 + 24h + 80 AChE 80 IgG AChE 50 IP : Cav-1 IgG 50 IP: Cav-1 80 AChE E40 0 8 16 24 48 72 h 80 AChE IgG 50 si Cyt c IP: Cyt c IgG 50 IgG IP: Cav-1 50 25 Cyt c IP: Apaf-1 E50 (LNCaP) E10 (PC-3) PC PC 0 24 48 h 0 24 48 h IP: Apaf-1 WB: AChE IP: AChE WB: AChE IP: Apaf-1 WB: AChE IP: AChE WB: AChE IgG IgG IgG IgG IP: Cav-1 WB: AChE IP: AChE WB: AChE IP: Cav-1 WB: AChE IP: AChE WB: AChE IgG IgG IgG IgG IP: Cyt c WB: AChE IP: AChE WB: AChE IP: Cyt c WB: AChE IP: AChE WB: AChE IgG IgG IgG IgG IgG IgG IgG IgG IP: Cyt c WB: Apaf-1 IP: Apaf-1 WB: Apaf-1 IP: Cyt c WB: Apaf-1 IP: Apaf-1 WB: Apaf-1 Supplementary Fig. S3 B A C D E40 0 8 16 24 48 h PC IP: Apaf-1 WB: Apaf-1 Apaf-1 105 IgG IgG 50 IgG IgG 15 IP: Cyt c WB: Cyt c Cyt c 50 IP: Cav-1 E

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