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vaccinia-T7

VSV REVERSE GENETICS. T7. T7. T7. T7. N. P. L. template. viral proteins. T7 polymerase. vaccinia-T7. ...UUG. AAA. UUGUCNNAG. N. P/C. M. G. L. 47. 1333. 821. 838. 1672. 6380. 59. COPY-BACK. DI TEMPLATES. VSV RNA SYNTHESIS. leader-N gene junction. other gene junctions.

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vaccinia-T7

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  1. VSV REVERSE GENETICS T7 T7 T7 T7 N P L template viral proteins T7 polymerase vaccinia-T7

  2. ...UUG AAA UUGUCNNAG... N P/C M G L 47 1333 821 838 1672 6380 59 COPY-BACK DI TEMPLATES VSV RNA SYNTHESIS leader-N gene junction other gene junctions G ...AUACU A UUGUCNNAG... C 7 z a 5' 3' (—) a z 3' 5' (—) TRANSCRIPTION REPLICATION a' z' leader 5' 3' P/C A (+) n A G z n a N A M n L 3' 5' A n (—) A n z z' 5' 3' (+) leader = N > P/C > M > G > L z' z 3' 5' (—)

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