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Transcription in E.coli by the numbers

Transcription in E.coli by the numbers. Size of typical gene = 1kb Typical number of mRNAs = 10 Typical rate of mRNA production = 10 min -1 Typical mRNA degradation rate = 1 min -1. mRNA production in E.coli. Golding et al. ‘05. r=1/7min ° =ln2/50min.

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Transcription in E.coli by the numbers

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  1. Transcription in E.coli by the numbers • Size of typical gene = 1kb • Typical number of mRNAs = 10 • Typical rate of mRNA production = 10 min-1 • Typical mRNA degradation rate = 1 min-1

  2. mRNA production in E.coli Golding et al. ‘05 r=1/7min °=ln2/50min

  3. Master equation for mRNA production Master equation for p(m,t): Poisson distribution with mean Steady state:

  4. Variance of the mRNA number var(m) = 4 <m> Poisson: var(m) = <m> Poisson Inconsistent with unregulated transcription model

  5. Single cell trajectories ¢n Transcription occurs in bursts!

  6. Genetic switch Gardner, Cantor and Collins ‘00

  7. Flipping the genetic switch Bistability!

  8. Nullclines and flow field

  9. Phase portrait of the genetic switch

  10. Repressilator Elowitz and Leibler ‘00

  11. The Poisson distribution What is the distribution of people per square? Histogram Poisson Probability people/unit area

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