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Improvement of Rainfed Lowland Rice Variety for Cooking Quality Traits using MAS

Improvement of Rainfed Lowland Rice Variety for Cooking Quality Traits using MAS. Sureeporn Katengam Uraiwan Kotchasatit UBONRATCHATHANI, THAILAND. Background. Rice production in NE of Thailand Area of production ranging from drought-prone lands to flooding area

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Improvement of Rainfed Lowland Rice Variety for Cooking Quality Traits using MAS

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  1. Improvement of Rainfed Lowland Rice Variety for Cooking Quality Traits using MAS Sureeporn Katengam Uraiwan Kotchasatit UBONRATCHATHANI, THAILAND

  2. Background • Rice production in NE of Thailand • Area of production ranging from drought-prone lands to flooding area • Abiotic stresses related with unpredictable rainfall pattern are main problems

  3. State of problem • Abiotic stresses associated with unpredictable rainfall pattern are main problems for rice production in NE of Thailand. • Elite cultivars resistance to drought are required.

  4. State of problem (cont.) • KDML105, an aromatic rice with good cooking quality, is widely grown in NE, however, its production is dominating constraint with the drought problem. • Drought tolerance is a complex traits since this trait is governed by many QTLs locating in many chromosome (ch1, ch2, ch3, ch4, ch7, ch8,and ch9). • Therefore, improvement KDML105 for drought resistance is not an easy tasks.

  5. State of problem (cont.) • In the opposite way, improving of well adapted variety to drought prone areas for other quality traits such as cooking quality is worth to reconsider. • Cooking quality trait is determined by three characters specifying the physical properties of starch in the endosperm: • Amylose content (AC) • Gel consistency (GC) • Gelatinization temperature (GT)

  6. Objectives • Marker-assisted backcross breeding is aimed at improving well drought adapted variety to rainfed lowland area of NE of Thailand for cooking quality traits utilizing tightly linked DNA markers. • The major QTL controlling AC, GC, and GT are located on Ch.6. DNA marker of flanking markers closely linked to this major QTL controlling quality traits will be carried out for MAS.

  7. Plant materials • Donor parent: • KDML105 • low AC (~16-17%):major QTL on ch6 (at Wx gene ) • low GT: major QTL on ch6 • medium GC: major QTL on ch6 (at Wx gene ) • Recipient parent: • IR57514-drought

  8. Breeding scheme:

  9. Genetic map (Azu / IR64 ) Chr.11 Chr.6 Map location of QTLs controlling cooking quality traits

  10. Survey of SSR markers for parent screening

  11. Screening parents with SSR markers PCR condition PSS II-1 PSS II-2 RM473E RM205 RM204 RM229 RM457 RM206 RM254 RM121 RM597 RM190 RM225 RM111 RM121 RM136 RM21 RM21 RM50 WAXY BADH GT11 726bp 1 2 3 553bp 500bp 427bp 311bp 249bp 200bp Thermal cycling 151bp 140bp 118bp 1 KD 2 IR57514 3 KD571-77 100bp

  12. SSR markers chosen for parents screening

  13. 2nd workshop on December MAS for BC1F1 Planting parents early-April donor recipient KD571-77 X IR57514 late-June mid-July F1 early-August Planting F1 and recipient parents mid-October F1 X IR57514 mid-November BC1F1 MAS Line selection with selected SSR markers late -November 1 2 3 4………………………………. .200 lines

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