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Clinical pathology: SEROMARKER FOR TB

Clinical pathology: SEROMARKER FOR TB. GENERAL OBJECTIVE : After finishing lab activity of Seromarker TB, the student will be able to describe the Seromarker TB in the DMS case problem SPECIFIC OBJECTIVE :

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Clinical pathology: SEROMARKER FOR TB

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  1. Clinical pathology: SEROMARKER FOR TB GENERAL OBJECTIVE: After finishing lab activity of Seromarker TB, the student will be able to describe the Seromarker TB in the DMS case problem SPECIFIC OBJECTIVE: At the end of lab activity of Seromarker TB, the student will be able to interprete: - the normal of Seromarker TB - the abnormal of Seromarker TB

  2. Introduction • Tuberculosis : Chronic infectious disease • involves multiple organs • >> lung M. tuberculosis • Indonesia: number 3 in the world • after India and China • West Java Health Profile 2000: • The caused of morbidity (no 4) in the hospital • Number 2 the caused of mortality

  3. Transmission • droplet nuclei size 1-5 µm 1-3 AFB • Source • of infection Patients with Sputum AFB (+)

  4. Diagnosis: - Direct smear:Ziehl Nielson: Acid fast bacilli - Culture: GOLD STANDARD - Tuberculin test - Chest X ray (Pulmonary TB)

  5. Problem? • Culture: 4-6 weeks • Not available in all center/laboratory • Extra pulmonary TB • Smear negative Pulmonary TB • Children SEROMARKER TB/Immunological test Detection of antibodies againts TB Mycobacterium tuberculosis

  6. Anti Myc. TB ELISA • ICT TB - A chromatographic immunoassay for qualitative detection of antibodies againts TB in human serum or plasma - The test employing recombinant antigen 38kD, 16 kD and 6 kD (ESAT-6), lipoarabinomannan - The use of a collection of antigen improves the sensitivity of serological test Seromarker TB

  7. Mycotec TB( recombinant) employs the complexes conjungate with gold colloidal particle passes over the immobilized multiple of recombinant TB ag precoated on the test area (T) once the patient sample is added to the sample well. Seromarker TB

  8. If there are some ab to TB are present in patient sample then the TB recombinant ag capture them, a pink/purple band is produced on the test area. Principle of the test

  9. The migration of remaining complexes which were not bound by those TB ab, will be then immobilized to the control area (C). • A pink/purple band is appeared on control area, the band on control performs a properly test

  10. Prosedur Pengujian 1. Pipetkan 100 μl serum atau plasma 2. Baca hasil 5-20 menit setelah penetesan spesimen Interpretasi Hasil PositifTampak 2 garis warna merah muda atau ungu di area Test (T) dan Kontrol (C) NegatifTampak 1 garis warna merah muda atau ungu di area Kontrol (C) InvalidTidak tampak garis di area Kontrol (C)

  11. Mekanisme Reaksi

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