1 / 18

Gel Electrophoresis

tessa
Download Presentation

Gel Electrophoresis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

    1. Gel Electrophoresis

    2. What is Gel Electrophoresis? Electrophoresis: Movement of charged particles through a solution (gel) under the influence of an electric field DNA fragments have a negative (-) charge and will move toward the positive electrode

    3. The Basic Components The “gel” in gel electrophoresis is the component that physically separates the molecules. The electric power supply provides the electricity that carries the molecules through the gel

    4. Gels are often made of a gelatin-like substance called agarose. A comb with teeth is suspended in the gel until it cools. The Process

    5. When the gel has cooled and solidified, the scientist pulls the comb out of the gel. The teeth of the comb leave impressions in the gel called wells where the DNA will be loaded.

    6. The gel is placed in the electrophoresis chamber. Gel acts like a bridge through which the molecules must travel to get from one side of the chamber to the next.

    7. DNA Preparation Restriction Enzymes Cut DNA into small pieces Restriction enzymes recognize specific sequences in the DNA molecule (for example GATATC) and cuts the DNA wherever that specific sequence occurs.

    8. Gels usually contain several different samples of DNA for study. The researcher uses a pipette to draw out a small, precisely measured amount of each sample and places each sample in its own well. Loading DNA Samples into the Gel

    13. Applications of Gel Electrophoresis DNA Criminal investigations Blood Saliva Hair Semen Paternity Tests

    14. Forbidden Donut Mystery Your Analysis Unit is charged to determine who took a bite of Homer’s Forbidden Donut. Posterboard = ? Names on the Posterboard = ? Scissors = ? DNA strips from suspects

    15. Forbidden Donut Mystery Your Analysis Unit is charged to determine who took a bite of Homer’s Forbidden Donut. Posterboard = GEL Names on the Posterboard = ? Scissors = ? DNA strips from suspects

    16. Forbidden Donut Mystery Your Analysis Unit is charged to determine who took a bite of Homer’s Forbidden Donut. Posterboard = GEL Names on the Posterboard = WELLS Scissors = ? DNA strips from suspects

    17. Forbidden Donut Mystery Your Analysis Unit is charged to determine who took a bite of Homer’s Forbidden Donut. Posterboard = GEL Names on the Posterboard = WELLS Scissors = RESTRICTION ENZYME DNA strips from suspects

    18. Forbidden Donut Mystery How to cut and place sequences on gel

More Related