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1. The Role of Genes AT5G48150 and AT2G04890in Arabidopsis thaliana Seed Development Jennifer Huynh
June 8, 2006
Honors Collegium 70AL
Professor Bob Goldberg
2. Overview
3. What has been already discovered about the genes?
4. The GRAS Family Name derives from the three initially identified members
GAI, RGA, and SCR
Functions range from meristem maintenance to regulation of hormone and light signal transduction
PAT1 branch plays role in far red light signaling
Homologs found in higher plants
Tomato, petunia, lily, rice, barley
5. The GRAS Family Tree
6. The GRAS Family Name derives from the three initially identified members
GAI, RGA, and SCR
Functions range from meristem maintenance to regulation of hormone and light signal transduction
PAT1 branch plays role in far red light signaling
Homologs found in higher plants
Tomato, petunia, lily, rice, barley
7. Gene One: AT5G48150 Scarecrow-like transcription factor 21 (SCL21)
Gene Size: 2355 bp Protein Size: 491 aa
8. Gene Two: AT2G04890 Phytochrome A signal transduction 1 (PAT1)
Gene Size: 2601 bp Protein Size: 414 aa
9. Where is the gene transcribed into mRNA?
10. mRNA Accumulation: AT5G48150
11. mRNA Accumulation: AT2G04890
12. Is the upstream region of the gene responsible for the transcription of the gene?
13. Restriction-Digested Plasmid DNA
14. What happens if the genes are knocked out?
15. Genotyping Insertion Lines Wildtype: 1508 bp
Salk band: 756 bp
Found band: 880 bp
16. Genotyping Insertion Lines
17. AT5G48150: SALK_064220 Wildtype: 1796 base pairs
Salk band: 1740 base pairs
Found band: 1739 base pairs
18. AT2G04890: SALK_146085 Wildtype: 1508 base pairs
Salk band: 756 base pairs
Found band: 880 base pairs
19. Nomarski Photographs
20. Conclusions No mutant phenotype on flowers, leaves, or siliques for either AT5G48150 or AT2G04890
Possibilities
Genes not vital for seed development
Similar genes exist that compensate
Mutant phenotype not visible in experiments
More research is necessary
21. Future Research Perform RT-PCR on homozygous mutants seeds to observe transcription
Fuse GFP marker with promoter to observe expression
Cross homozygous mutant plants of different knockouts
22. Acknowledgements Bob Goldberg
Anhthu Bui
Mike Gavino
Jonathan Russell
Ria Yagnik Brandon Le
Xingjun Wang
Tomo Kawashima
Jessica Luke
HC 70AL Classmates