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Importance of microRNA for seedling development in Arabidopsis thaliana

Importance of microRNA for seedling development in Arabidopsis thaliana. Jennifer Coppersmith Department of Biochemistry and Biophysics Mentor: Dr. Hiro Nonogaki (Horticulture). Significance of Seed Development. Agriculture and Food Why Arabidopsis?. pgrc3.agr.ca/images/seeds.gif.

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Importance of microRNA for seedling development in Arabidopsis thaliana

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  1. Importance of microRNA for seedling development in Arabidopsis thaliana Jennifer Coppersmith Department of Biochemistry and Biophysics Mentor: Dr. Hiro Nonogaki (Horticulture)

  2. Significance of Seed Development • Agriculture and Food • Why Arabidopsis? pgrc3.agr.ca/images/seeds.gif

  3. Testa Endosperm Embryo Arabidopsis Seed Structure

  4. T DNA insert Overview of Experiment SPL13 SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13)

  5. microRNA • 21-24nt in length • Gene regulation at the mRNA level www.qiagen.com/about/PressReleases/images/9.jpg

  6. mRNA miRNA miRNA RISC RISC microRNA

  7. SPL13 miR156 miRNA mSPL13 A A A A RISC microRNA mutation Mutation mRNA Hypothesis: SPL13 needs to be down regulated by microRNA156 to maintain normal developmental program for germination or seedling establishment.

  8. Overaccumulation of SPL13 WT Cont mSPL13 SPL13 rRNA

  9. Cot Cot 2 leaf seedling (delayed) Mutant Phenotype Cot Cot 4 leaf seedling (normal)

  10. Developmental Delay 4-leaf seedlings 2-leaf seedlings

  11. WT mSPL13 CONTROL Temporal Seedling Arrest

  12. 2 d Shoot Apical Meristem (SAM) 0 d

  13. Meristem Cot Embryo Storage Protein and Meristem Hypocotyl Radical

  14. 3 d 2 d 4 d WT mSPL13 1 d 2 d 3 d 4 d mSPL13 mSPL13 mSPL13 WT WT WT Meristem Development

  15. Visible Difference WT mSPL13 8 d

  16. Analysis WT mSPL13 SPL13 Based on phenotype, down regulation of SPL13 via microRNA is essential for proper meristem development.

  17. Promoter Gene X X Determine Potential Targets of SPL13 miRNA156 SPL13 SPL13

  18. Acknowledgements Hiro Nonogaki Kevin Ahern Howard Hughes Medical Institute Lab Members: Masa Asahina Tammy Chan Po-Pu Liu Jessica Kristof Piotr Pupel Jing Sun

  19. SBP1 SBP2 SPL13 ? SQUAMOSA ( or APETALA1) - 455 - 429 SQUA AP1 - 419 - 393 10 bp conserved stretch 19-mer core seq

  20. Normal Embryogenesis in mSPL13 Coppersmith JL, Hewitt JR, Nonogaki H, unpublished results

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