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Laboratory of Microbial Ecology and Technology (LabMET) Ghent University Belgium Nico Boon

Laboratory of Microbial Ecology and Technology (LabMET) Ghent University Belgium Nico Boon. Research topics and expertise. Ghent: Population: 250,000 Established: 630 A.D. In 1350: Largest city of W Europe after Paris Historical and cultural richness. Where is Ghent situated?.

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Laboratory of Microbial Ecology and Technology (LabMET) Ghent University Belgium Nico Boon

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  1. Laboratory of Microbial Ecology and Technology (LabMET) Ghent University Belgium Nico Boon Research topics and expertise

  2. Ghent: Population: 250,000 Established: 630 A.D. In 1350: Largest city of W Europe after Paris Historical and cultural richness Where is Ghent situated?

  3. GhentUniversity • Founded in 1817 by William I, King of the Netherlands • 1990’s - today: uninterrupted growth • 11 faculties, education and research in all scientific branches • 32.000 students and 7.100 staff members • Open, committed and pluralistic university • Broad international perspective • Located in the cultural and economical heart of Europe

  4. Faculty – CES – Department • Faculty of Bioscience Engineering • Now: 1600 Bioengineer students • BaMa structure • Bachelor (3 yr.) + Masters (2 yr.) • Centre forEnvironmentalSanitation • International program for masters • Each year 10 scholarships (300 applicants) • See website: www.cms.ugent.be • Department Biochemical and Microbial Technology • Lab. Industrial Microbiology and Biocatalysis • Lab. Microbial Ecology & Technology

  5. Laboratory of Microbial Ecology and Technology • Head of department: Willy Verstraete • Teaching staff:N. Boon, T. Van de Wiele, K. Rabaey (09/11) • Post-doc: 9 • Scientific staff: 25 PhD-collaborators • Technical staff: 8 persons • Administrative staff: 3 persons • Master students: 20

  6. Microbes as multicellular organisms LabMET mission statement « Study mixed microbial communities & interactions to better understand ecological processes and use and/or steer the microbial community in biotechnological applications »

  7. Main research areas • Applied Microbial Ecology (community/stability) • New ecological theories • New tools and analysis • Microbial Resource Management • Environmental biotechnology (water & soil) • (Micro)-pollutant clean up • Methane production/removal • Microbial based materials • Bio Electrochemical Systems (BES) • Gastro-intestinal microbiology • Food/Feed/Probiotics/Prebiotics • Bacteria-host interactions

  8. Involvement in ULIXES Two work packages • WP4 (13 PM) • Task 4.3. Collection 3. • Biopalladiumnanoparticles precipitating prokaryotes • WP7 (26 WP) • WP leader • Task 7.3. Development of novel biopalladium nanoparticles dechlorination processes

  9. Biological production of nanoparticles M+ M+ M+ M+ M+ M+ M(0) Bacterium (1m) Metal salt Reducing agent Capping agent

  10. Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 Pd0 10 nm PdII PdII PdII PdII PdII 1 µm Pd0

  11. Production of Pd nanoparticles = BioPAD NaCOOH CO2 Pd(0) + Pd(II) • Bacterial cells couple the reduction of soluble Pd(II) to the oxidation of an electrondonor • Deposition of this biogenic Pd as nanoparticles on the cell wall and periplasmaticspace

  12. Shewanellaoneidensis(De Windt et al., 2005; Environ Biotechnol, 90:377-389;LabMET)

  13. Application of BioPAD as catalyst Cl Cl Cl H H + H2 H H H H H H H H H H H H • Loading the bio-Pd with H2/formate=> strong reductive capacities • Applicable for chlorinated solvents, PCB’s, dioxines, pesticides (lindane), nitrate, perchlorate, EE2, diatrizoate + Cl-

  14. Cl Cl Cl Cl Cl Cl H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H Bio-palladium is catalytic: PCBs + H+ + Cl- + H+ + Cl- + H+ + Cl-

  15. PCB contaminated sediment No BioPAD 50 mg/L BioPAD 500 mg/L BioPAD De Wind et al 2005 Environmental Microbiology, 7, 314–325 -LabMET

  16. Tasks and challenges within ULIXES Isolation of “collection 3” in WP4 (Task 4.3): • New metal precipitating strains from marine environments • Metal Precipitating Marine Bacteria: MP-MB • In situ or ex situ production of catalysts • New hydrogen forming bacteria from marine environments • Hydrogen Producing Marine Bacteria: HP-MB • In situ activation of the MP-MB • Experimental procedures • Screening in batch for Pd precipitating bacteria from partners • Characterisation (SEM, TEM, EDX, …) • Screening in batch for hydrogen producing bacteria from partners • Focus on activity and substrate specificity

  17. Tasks and challenges within ULIXES Development of PCB dechlorination product in WP7 (Task 7.3): • Marine environmental applications never explored • Catalyst poisoning • Activation of Pd in the marine systems • Proof of principle using H2 gas • Using in situ biogenic H2 (backup: MFC based H2 production) • Recovery and release of Pd in the environment • Background values in the sediments of Pd? • Immobilisation of Pd (polymers) • Recovery of Pd

  18. Recent research in relation to Ulixes Production and characterization of biological palladium • Chidambaram, D., Hennebel, T., Taghavi, S., Mast, J., Boon, N., Verstraete, W., van der Lelie, D., & Fitts, J.P. (2010). Concomitant microbial generation of palladium nanoparticles and hydrogen to immobilize chromate. Environmental Science & Technology, 44, 7635-7640. • De Windt, W., Boon, N., Van den Bulcke, J., Rubberecht, L., Prata, F., Mast, J., Hennebel, T., & Verstraete, W. (2006). Biological control of the size and reactivity of catalytic Pd(0) produced by Shewanellaoneidensis. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 90, 377-389. Catalytic action of biological palladium in reactors • Forrez, I., Carballa, M., Fink, G., Wick, A., Hennebel, T., Vanhaecke, L., Ternes, T., Boon, N., & Verstraete, W. (2011). Biogenic metals for the oxidative and reductive removal of pharmaceuticals, biocides and iodinated contrast media in a polishing membrane bioreactor. Water Research, 45, 1763-1773. • Hennebel, T., Benner, J., Clauwaert, P., Vanhaecke, L., Aelterman, P., Callebaut, R., Boon, N., & Verstraete, W. (2011). Dehalogenation of environmental pollutants in microbial electrolysis cells with biogenic palladium nanoparticles. Biotechnology Letters, 33, 89-95. • Hennebel, T., De Corte, S., Vanhaecke, L., Vanherck, K., Forrez, I., De Gusseme, B., Verhagen, P., Verbeken, K., Van der Bruggen, B., Vankelecom, I., Boon, N., & Verstraete, W. (2010). Removal of diatrizoate with catalytically active membranes incorporating microbially produced palladium nanoparticles. Water Research, 44, 1498-1506. • Hennebel, T., De Gusseme, B., Boon, N., & Verstraete, W. (2009). Biogenic metals in advanced water treatment. Trends in Biotechnology, 27, 90-98. • Hennebel, T., Simoen, H., De Windt, W., Verloo, M., Boon, N., & Verstraete, W. (2009). Biocatalytic dechlorination of trichloroethylene with bio-palladium in a pilot-scale membrane reactor. Biotechnology and Bioengineering, 102, 995-1002. • Hennebel, T., Simoen, H., Verhagen, P., De Windt, W., Dick, J., Weise, C., Pietschner, F., Boon , N., & Verstraete, W. (In press). Biocatalytic dechlorination of hexachlorocyclohexane by immobilized bio-Pd in a pilot scale dynamic bed reactor. Environmental Chemistry Letters. • Hennebel, T., Verhagen, P., Simoen, H., De Gusseme, B., Vlaeminck, S.E., Boon, N., & Verstraete, W. (2009). Remediation of trichloroethylene by bio-precipitated and encapsulated palladium nanoparticles in a fixed bed reactor. Chemosphere, 76, 1221-1225

  19. UGent Researchers on Ulixes • Nico Boon • Group leader • One person to hire (2011-2014) • Post-doc on Ulixes • Daily research and management • Tom Hennebel • Post doc; synthesis and applications of Bio-Pad • Bart De Gusseme (2011) • Phd student; reductive removal of micropollutants • Simon De Corte (2011-2013) • Phd student; new types of biogenic nanocatalysts (bimetallic particles) • SynthiaMaes and SteffiePardaens (2011-2012) • Master students

  20. Extra possibility within ULIXESContinuous High Pressure Reactor BioresourceTechnology, 2010, 101, 3132-3138.

  21. Acknowledgements Ir. David van der Ha (PhD) Ir. Bert Bundervoet (MSc) GwynetLeyre (MSc) Prof. Dr. ir. Willy Verstraete More information: www.labmet.Ugent.be CeesBuisman: WETSUS, Leeuwarden and Wageningen University, The Netherlands Lars Angenent: Cornell University, USA Ruth Ley: Cornell University, USA Nathalie Delzenne: UniversitéCatholique de Louvain, Belgium JeroenRaes: Free University of Brussels, Belgium Tom Curtis: Newcastle University, UK Marc Strous: Max Planck Institute for Marine Microbiology, Germany Mark Van Loosdrecht: Delft University of Technology, The Netherlands Ken Timmis: Braunschweig University of Technology, Germany Willy Verstraete: Ghent University, Belgium • www.LabMET.Ugent.be

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