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AP Lab 4: Diffusion and Osmosis

AP Lab 4: Diffusion and Osmosis. November 18-23. Order. Wednesday – part A (PMPH A/B due) Thursday – part B, set up D Friday – D final masses, C (PMPH C/D due) Monday – class data, Prelab Graph due Tuesday – class data Monday - due. Part A. H1: Which sized cell will diffuse faster?

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AP Lab 4: Diffusion and Osmosis

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  1. AP Lab 4: Diffusion and Osmosis November 18-23

  2. Order • Wednesday – part A (PMPH A/B due) • Thursday – part B, set up D • Friday – D final masses, C (PMPH C/D due) • Monday – class data, Prelab Graph due • Tuesday – class data • Monday - due

  3. Part A H1: Which sized cell will diffuse faster? H0: Size doesn’t matter – SA:V ratio has no effect on diffusion • Purpose is to see how SA:V ratios affect diffusion in a cell • Basic Procedure: • Cut out 3 sized agar cubes • Calculate SA:V ratio • Make predictions of fastest diffusion • Place into beaker of NaOH for 10 minutes. • Blot and cut in half • Measure depth of diffusion

  4. Part B H1: Which materials will go through the membrane? H0: No materials will go through the membrane. • Purpose is to see what substances go through a dialysis tube • Basic Procedure • In tube: 15 ml glucose/starch soln • Beaker: distilled water and 4 ml of IKI • Qualitative data before and after 30 minutes

  5. How to open a dialysis bag How to open a dialysis bag

  6. Part C • Purpose is to see the effects of different concentrations of sucrose solutions on dialysis tubing • Basic Procedure • 5 dialysis tubes  25 ml of Om, 0.2M, 0.4M, 0.6M and 0.8M sucrose solutions • Initial mass • Place in distilled water for 30 minutes • Final mass, % change in mass, class averages, graph and standard error calculations and put on graph H1: Which tube will lose/gain most mass? H0: different sucrose solutions don’t affect osmosis in a dialysis tube.

  7. H1: Which potato will have the highest/lowest water potential? Ho: All three potatoes have the same water potential. Part D • Purpose is to determine the water potential of 3 different potatoes. • Procedure: 5 pieces of each potato and get initial mass • Put each potato in each solution: 0M, 0.2M, 0.4M, 0.6M and 0.8 M (color coded) • Final Mass • Figure out which color is which solution • Percent change in mass, class averages, graph, figure out molarities of each potato • Calculate water potential

  8. Part E • Purpose is to observe hypertonic, isotonic and hypotonic environments with a plant cell • Basic Procedure: • Elodea normal water – high power • Elodea distilled water – high power • Elodea salt water – high power • Draw each and label in notebook

  9. Inquiry • Purpose is to predict and see what will diffuse through a dialysis bag using various substances. • Materials available: • 1M NaCl solution • 1M Sucrose solution • 1M glucose solution • 5% albumin solution (egg white solution) • 0M distilled water

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