1 / 15

UV-B Radiation, Poplar Phytochemistry & Herbivory

UV-B Radiation, Poplar Phytochemistry & Herbivory. Jeffrey M. Warren John H. Bassman Washington State University EPA STAR Graduate Fellowship Program. Ultraviolet Radiation. Ozone Loss UV-B Radiation Increase Chromophores Phenolics, proteins, DNA Effects Direct versus indirect.

theta
Download Presentation

UV-B Radiation, Poplar Phytochemistry & Herbivory

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. UV-B Radiation, Poplar Phytochemistry & Herbivory Jeffrey M. Warren John H. Bassman Washington State University EPA STAR Graduate Fellowship Program

  2. Ultraviolet Radiation • Ozone Loss • UV-B Radiation Increase • Chromophores Phenolics, proteins, DNA • Effects Direct versus indirect

  3. Effects of UV-B Radiation

  4. Objectives • Identify effects of enhanced UV-B radiation on Populus tricocarpa foliar biochemistry: PS, chlorophylls, flavonoids, nutrients, tannin • Do these UV-B induced changes affect Chrysomela scripta herbivory: Growth rate, preference

  5. Methodology - UV-B Lamps • Enhanced UV-B lamp systems Indoor non-modulated, Outdoor modulated • Indoor Study Design 3 treatments (0X, 1X, 2X UV-B for Pullman, WA) 4 blocks 4 trees/block

  6. Methodology - Trees • Harvest Parameters Growth, leaf area, leaf plastochron index • Chemistry Photosynthesis - 14C (30 sec pulse) Chlorophyll a, b - DMF extraction Flavonoids - HPLC (ether, EA purification) Nitrogen, Sulfur, Carbon - Combustion Tannins - CBB-BSA binding assay

  7. Methodology - Insects • Feeding preference trials Single beetle in petri dish with leaf disks 3 treatments, 30 reps, 24 hours • Growth rate trials Recently hatched larva grown on single leaf 3 treatments, 90 reps, 2-3 weeks

  8. Conclusions • Increased UV-B radiation: • Increased foliar nitrogen and photosynthesis • Increased flavonoids but decreased tannins • Increased CLB preference / CLB growth rate

More Related