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Basic parametersApplicationsCell phenotypingMeasurement of cell activationVisualization of cell divisionDetection of intracellular molecues (e.g. cytokine)Analysis of specific ligand bindingAnalysis of intracellular calcium concentrationAnalysis of DNA content Cell sorting. Applications o
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1. Applications of flow cytometry in basic immunology
Generation and characterization of DC
Assays for T cell activation
Cell proliferation
Cell division
Cell activation marker Laboratory demonstration - DC and T cell activation
2. Basic parameters
Applications
Cell phenotyping
Measurement of cell activation
Visualization of cell division
Detection of intracellular molecues (e.g. cytokine)
Analysis of specific ligand binding
Analysis of intracellular calcium concentration
Analysis of DNA content
Cell sorting Applications of Flow Cytometry in Basic Immunology
3. FSC (forward scatter) - Size
SSC (side scatter) - Granularity
FL (flurochrome) FL-1, FL-2, FL-3 etc. Flow cytometry - the basic parameters
4. (1) FSC the size