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I. Ia. Ib. II. III. IV. V. VI. N. T-aGKT. Y--p-kal-nq. -TtE. I-DE-H. SaT/S. ivFv-sr. T-Tla-GvN-PA. QM-GRAGR-g. Consensus. N. TssGKT. YttPlKALsNQ. -TTE. vlDEvH. SAT. IwFifnR. TeTLAaGiNmPA. QMaGRAGRrG. ISE2. N. TsAGKT. YtaPiKtisNQ. -TTE. IfDEvH. SAT. vVFcfSk.
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I Ia Ib II III IV V VI N T-aGKT Y--p-kal-nq -TtE I-DE-H SaT/S ivFv-sr T-Tla-GvN-PA QM-GRAGR-g Consensus N TssGKT YttPlKALsNQ -TTE vlDEvH SAT IwFifnR TeTLAaGiNmPA QMaGRAGRrG ISE2 N TsAGKT. YtaPiKtisNQ -TTE IfDEvH SAT vVFcfSk TeTfAmGvNaPA QMaGRAGRrG SKI2p Supplemental Figure S2. Top: Comparison of consensus DEVH RNA helicase motifs to such motifs in ISE2 and Ski2P from yeast.Adapted from N. Kyle Tanner and Patrick Linder, Molecular Cell, Vol. 8, 251–262 (2001) Bottom: Phylogenetic tree of ISE2 homologs in diverse organisms using the neighbour-joining BLOSUM 62 matrix (Blastp) and represented graphically using Jalview software. Number in each branch indicates the distances. NP_177164 is ISE2 Arabidopsis.