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Hind III

Hind III. EcoR I. EcoR I. Restriction Map 1 Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. EcoR I = 2 sites Hind III = 1 site. 10k. 10k. 1K. 10k. 7k. 1.) Linear or Plasmid ?. =10K. 2.) What’s the Size?. 6K. 10K. = 3 sites.

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Hind III

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  1. HindIII EcoRI EcoRI Restriction Map 1Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. EcoRI = 2 sites HindIII = 1 site 10k 10k 1K 10k 7k 1.) Linear or Plasmid ? =10K 2.) What’s the Size? 6K 10K = 3 sites 3.) How many cuts? Linear: Fragments = cuts + 1 Plasmid : Fragments = cuts 3k =HindIII 4.) Easy Cuts First! 5.) Find relationships! 10k 6.) Rotate

  2. HhaII XhoI Restriction Map 2Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. HhaII = 1 site XhoI = 1 site 2K 4K 6K

  3. Restriction Map 3 A RIVER runs from Stanton to Fredericksburg. Two Cabins are located on this stretch of river. The Teelin’s cabin and the Larson’s cabin are 5 km apart. The Larson’s cabin is 8 km from Stanton while the Teelin’s cabin is 11 km from Fredericksburg. Using the data, make a map of the river and the cabins located along it. Use a horizontal free line to represent the river and symbols to represent the cabins. Label the symbols and intervals between symbols with the measurements of distance between the cabins. • How far is the Teelin’s cabin to Stanton? • How far is the Larson’s cabin to Fredericksburg? • Is there an alternate Map?

  4. 8 km 11 km Stanton Teelin’s 5 km Larson’s Fredericksburg 11 km 8 km 5 km Stanton Teelin’s Larson’s Fredericksburg 13Km How far is the Teelin’s cabin to Stanton? How far is the Larson’s cabin to Fredericksburg? Is there an alternate Map? 16Km You Betcha , See Below! 3Km Now how far is the Teelin’s cabin to Stanton? Now how far is the Larson’s cabin to Fredericksburg? 6Km

  5. Restriction Map 4 There are towns along highway 28 running east to west. Using the data below, make a map of highway 28 and the towns located along it. Use a free horizontal line to represent highway 28 and symbols to represent towns. Label the intervals between the towns with the measurements of distance. • Freeville : Mt. Crumpit 160 km • Mt. Crumpit : Whoville 200 km • Waterville : Freeville 120 km • Whoville : Waterville 240 km • Mt. Crumpit : Waterville 40 km • Freeville hunters get to start each hunting day 8 min. sooner than Waterville hunters.

  6. West East 120K 40K 200K Freeville Whoville Mt. Crumpit Waterville

  7. SamIII TrpII 17K 10K Restriction Map 5Construct a Linear restriction map of the following digest. Include cuts and fragment sizes. SamIII = 2 sites TrpII = 1 site 27K 27K 13K 27K SamIII 10K 3K 5K 9K

  8. MtGI CabII MtGI CabII CabII Restriction Map 6Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. CabII = 3 sites MtGI = 2 site 1,500 10K 500 2K 10K 500 10K 4,500 10K 2,500 8K 1,000

  9. Restriction Map 7 • There is a trail that runs along a mountain ridge in eastern Vermont from James Camp to Camp Five. Using a horizontal line to represent the trail and dots to represent climbing features. Label the intervals between climbs with the measured distance. • Blood Lake : Washington Peak 3 km • Washington Peak : Misery Rock 4.5 km • Hangman's Cliff : One Way Jacks Trail 1 km • James Camp : Jackson's Thrill 2 km • Hangman’s Cliff : Misery Rock 9.5 km • Washington’s Peak : Jackson’s Thrill 4 km • Jackson’s Thrill : Misery Rock .5 km • One Way Jacks Trail : Jackson’s Thrill 10 km • Blood Lake : Camp Five 6.5 km • Jackson’s Thrill should be climbed in the sun before the sun sets behind Washington’s Peak

  10. 1.5K .5K 4K 3K 2K 1K 3.5K Camp V Blood Lake Misery Rock James Camp Hangman’s Cliff Washington’s Peak Jackson’s Thrill One Way Jack’s Trail

  11. Cow Person Pirate Superhero samurai

  12. Problem set 1: #1 EcoRI 2K 10K 8K BamHI

  13. Problem set 1: #2 EcoRI BamHI 2K 4K 12K 6K EcoRI

  14. Problem set 1: #3 EcoRI BamHI 1K 4K 12K 500 6.5K EcoRI BamHI

  15. Problem set 1: #4 ClaI =2 sites HindIII = 1site HindIII ClaI ClaI 7000 8000 2000 4000

  16. Problem set 1: #5 BamHI =1 site HindIII = 2 sites BamHI HindIII HindIII 15000 3000 7000 6000

  17. Problem set 1: #6 PvuI = 4 sites SmaI = 4 sites SmaI PvuI SmaI SmaI SmaI PvuI PvuI PvuI 5500 2800 3200 8000 4500 2000 2200 3000 3800

  18. Problem set 2: #7 BamHI EcoRI 1,500 BamHI 500 4,500 2,500 EcoRI =3 sites 1,000 BamHI = 2site EcoRI EcoRI

  19. 3,500 4,500 1,800 2,200 HindIII HindIII Problem set 2: #8 BamHI BamHI EcoRI EcoRI EcoRI 8,000 9,000 1,000 5,000 1,500 4,000

  20. Problem set 2: #9 EcoRI =2 sites BamHI = 1site BamHI EcoRI EcoRI 3,000 6,000 1,000 8,000

  21. Problem set 2: #10 BamHI ClaI HindIII EcoRI 6,000 5,000 3,000 5,000 4,000

  22. BkaI SnaTII 15,000 3,000 13,000 Quick Draw Δ(linear) 31,000

  23. ZtoPI TtHII 500 8,500 8,000 Quick Draw Σ(Plasmid)

  24. SpaZIII HmmI HmmI 100 10 30 Quick Draw Ω(linear) 15 155

  25. 8,500 500 RamII 2,000 RamII PhaTI Quick Draw β(Plasmid) 11,000

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