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The 6420 Triple Quad LC/MS System

The 6420 Triple Quad LC/MS System. Routine, Robust and Reliable Quantitation. Agilent has the Newest Fleet of LC Triple Quads. A New Agilent LC Triple Quad Launched Every Yr For last 4 yrs. 6490 Triple Quad. 6420 Triple Quad. 6460 Triple Quad. 6460 Triple Quad. Performance.

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The 6420 Triple Quad LC/MS System

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  1. The 6420 Triple Quad LC/MS System Routine, Robust and Reliable Quantitation

  2. Agilent has the Newest Fleet of LC Triple Quads A New Agilent LC Triple Quad Launched Every Yr For last 4 yrs 6490 Triple Quad 6420 Triple Quad 6460 Triple Quad 6460 Triple Quad Performance 6430 Triple Quad

  3. 6420 Enhancements over Earlier 6410 • New Capillary front end: faster polarity switching better ion transmission • New electronics, faster MRMs • New Agilent rough pump • Upgradeable to a 6460 with Agilent Jet Stream Technology 6420 Triple Quad LC/MS

  4. 6420: Fast Electronics for High Sample Throughput The Agilent 6420 Triple Quad features fast and rugged electronics; Making it a workhorse for high throughput labs Fast MRMs , dwell time of: 1 msec Fast polarity switching : 30 msec Fast Triggered MRM (for ten ion transitions): 50 msec

  5. Future Proof Your LC Triple Quad Investment Performance Step 2 6430 6460 Ultra-Trace Analysis 6420 Step 1 Trace Analysis Infinity LC Routine Analysis

  6. 6420 Upgrade Possibilities S/N Spec 8X 6460 Triple Quad 6430 Triple Quad 2X 6420 Triple Quad 1X

  7. New 6420 LC Triple Quad Supports: Agilent Upgrade Kits to 6430 and 6460 LC Triple Quads Agilent Dynamic MRM Agilent Fast Track Kits and Data Bases Agilent Triggered MRM

  8. Upgradeable by Design – 6400 Upgrades 6420 to 6430 Upgrade Improves Signal-to-Noise up to two-fold for some compounds The 6420 is preconfigured to accept a second turbo pump for enhanced sensitivity. Higher vacuum in Q3 reduces noise and allows greater ion conductance in the collision cell.

  9. 6430 to 6460 Add: Agilent Jet Stream Technology thermal confinement of ESI spray cone 6430 to 6460 Upgrade Sensitivity Spec: 4X higher 25 0C 350 0C after 8 min

  10. 6430 to 6460 Upgrade: High Mass Quads – 3K The 6460 mass range is 3,000 m/z: Pegylated peptides Acylated peptides Lipopolysaccharides Glycans Natural Products

  11. Dynamic MRM groups MRMs in Retention Windows Dynamic MRM creates powerful quantitative methods by grouping MRMs in retention time windows instead of time segments. Dynamic MRM methods are created by importing compound specific MRMs and retention times from a Fast Track Data Base or worksheet Dynamic MRM supports up to 4,000 MRMS for all 6400 Series Triple Quads Dynamic MRM also supports polarity switching

  12. Dynamic MRM for the new 6420 Triple Quad Retention Time Windows with overlapping MRMs 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 9 9 10 10 11 11 12 12 13 13 14 14 15 15 16 16

  13. Agilent Fast Track Kit for Forensics Includes a Dynamic MRM method, 200 compound data base with optimal settings and RTs FORENSIC/TOXICOLOGY TEST MIX – 24 compounds Compound Name Precursor Ion Product Ion Collision Energy Ret Time Hydrocodone 300.2 199 29 1.4 methamphetamine 150.1 119 65 1.45 MDMA 194.1 163 9 1.468 Strychnine 335.2 184 41 1.629 MDEA 208.1 163 9 1.735 …… …… ……

  14. Forensics Test Mixture on the new 6420 25 pg of each on-column Metham-phetamine Amphetamine Verapamil Trazodone Norver-apamil Benzoyl-ecgonine Methadone PCP Cocaine Proadifen 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2 Acquisition Time (min)

  15. Triggered MRM (tMRM) – a Data Dependent Scan Data dependent execution of additional, confirmatory MRMs 209.1 165.1 152.1 triggered MRM Spectra 77.0 105.0 219.0 60 100 140 180 220 m/z 2-Ethylanthraquinone

  16. Triggered MRM in MassHunter Acquisition Triggered cycle (above threshold) Additional MRMs used for compound confirmation Threshold Primary cycle (below threshold)

  17. tMRM Product Ion Spectrum on any 6400 TripleA Re-Constructed Product Ion Spectrum that is Library Searchable 334 > 119 334 > 117 334 > 105 334 > 91 334 > 76 x103 x103 x103 x103 x103 117.0 8 7 6 5 4 3 2 1 0 8 7 6 5 4 3 2 1 0 8 7 6 5 4 3 2 1 0 8 7 6 5 4 3 2 1 0 8 7 6 5 4 3 2 1 0 76.0 119.0 91.1 105.1 80 80 100 120 80 100 120 80 100 120 80 100 334 > 171 145.0 334 > 147 334 > 145 334 > 132 x103 x103 x103 x103 8 7 6 5 4 3 2 1 0 8 7 6 5 4 3 2 1 0 8 7 6 5 4 3 2 1 0 8 7 6 5 4 3 2 1 0 147.0 132.1 171.1 100 140 180 100 140 100 140 180 80 100 120 140 160 180 tMRM Product Ion Spectrum Tebufenpyrad 145.0 x103 117.0 8 7 6 5 4 3 2 1 0 76.0 91.1 132.1 105.1 171.1 334.2 80 100 120 140 160 180 200 220 240 260 280 300 320 340

  18. Benefits of triggered MRM • More than ten times more sensitive than full scan MS/MS data • Searchable with NIST MS/MS 2.0 Library • Searchable with Agilent Accurate Mass MS/MS Library • Compatible with custom, user created libraries • MassHunter automatically creates custom triggered MRM library from standards

  19. Forensics Mixture with 6420 and tMRM Method Triggered MRM actuated on red peak Metham-phetamine Amphetamine Verapamil Trazodone Norver-apamil Benzoyl-ecgonine Methadone PCP Cocaine Proadifen 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2 Acquisition Time (min)

  20. Triggered MRM for Compound Confirmation Ref. Library Match Score = 95.1 168.1 1 6420 tMRM: Benzoylecgonine, 25 pg on column 0.8 0.6 77.0 105.0 0.4 0.2 290.1 @ 5ppb level: RSD=4.9% (N=3) 0 Peak Product Ion Spectra -0.2 105.0 -0.4 77.0 -0.6 -0.8 -1 168.1 -1.2 100 150 200 250 300 Library Reference Product Ion Spectra Mass-to-Charge (m/z)

  21. 6420 Summary DENVER (American Society for Mass Spectrometry conference), June 6, 2011 The new Agilent 6420 Triple Quad LC/MS System is a workhorse triple quad designed to be readily upgradable to the higher-performance 6430 and 6460 instruments. The new 6420 is more productive than the previous 6410 model with faster electronics and new software features. Our popular dynamic MRM solution for grouping ion transitions by retention time has been enhanced with the addition of triggered MRM for simultaneous compound confirmation. The Agilent 6420 is well suited for both routine and complex analyses such as toxicology and drugs of abuse testing. It is designed for round-the-clock operation to support labs running a large number of samples. The 6420 replaces Agilent's popular Model 6410 triple quad. The 6420 maintains method and data compatibility with existing 6410 methods. The 6420 can be easily incorporated into high-productivity laboratories with multiple 6410 systems.

  22. THANK YOU!

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