1 / 18

Toxicity of Automotive Runoff on Daphnia.

Toxicity of Automotive Runoff on Daphnia. Morgan Dutrow 10 th Grade Bellwood-Antis. Why I Chose this Experiment. I chose this experiment because…. Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments.

tress
Download Presentation

Toxicity of Automotive Runoff on Daphnia.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Toxicity of Automotive Runoff on Daphnia. Morgan Dutrow 10th Grade Bellwood-Antis

  2. Why I Chose this Experiment I chose this experiment because… • Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments. • Earlier in the year I had learned a little about Daphnia.

  3. Background Information • Brake fluid • Contains: Diethylene glycol (C4O3H) Windshield Washer Fluid • Contains: Methanol (CHO3H) • Antifreeze • Contains: Ethylene glycol (C2H6O2) Lethal Dose 50 in 96 hours

  4. Hypothesis Windshield washer fluid be the least lethal.

  5. Why I Chose Daphnia… • In our experiment we used the variety of daphnia known as Daphnia Magnum. • Daphnia being much smaller, would be more susceptible. Because they are a food source, this could then work its way up the ecosystem. • Daphnia are an indicator species.

  6. General Procedure • 40ml fluid with 4 daphnia • 3 trials of each dilution

  7. Making a dilution: Ex. 1/10 dilution • Water: 34ml • Automotive pollutant: 4ml • Food:2ml

  8. Recipes For My Dilutions Dilution Water Food Chemical 1/2 18 2 20 1/5 30 2 8 1/10 34 2 4 1/20 36 2 2 1/40 37 2 1 1/80 37 ½ 2 ½

  9. Experimental Design • Variable • the pollutant • Experimental Group • Antifreeze, Brake Fluid, and Windshield Washer Fluid • Control Group • Spring Water

  10. Experimental Design Contd. • Control Variables: Amount of… • Light • Food • Chemical • Water Others… • Temperature • Size of daphnia • Clean materials

  11. LD 50 • Daphnia died too close together

  12. Daphnia Lifetime (in minutes) inBrake Fluid

  13. Averages Daphnia Lifetimes of 3 Trials Note: All numbers are in minutes.

  14. Conclusion According to my data, I reject my hypothesis that Windshield Washer Fluid would be the least lethal to daphnia.

  15. Improvements • I would… • use more specific dilutions. • use different animals. • use more chemicals.

  16. Expanding my Project • I could… • test motor oil by using a larger aquatic animal. • test to see what affect making the water more acidic or basic would have.

  17. Bibliography • http://ijt.sagepub.com/cgi/content/abstract/23/1/55 1/24 • http://www.aehms.org/jaeh_3_2_sherry.html 1/24 • http://www.jtbaker.com/msds/englishhtml/e5125.htm 2/8 • https://fscimage.fishersci.com/msds/89493.htm 2/8 • http://www.atsdr.cdc.gov/tfacts96.html 2/10

More Related