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Mentor : Professor William C. Tang Grad Student : Yu-Hsiang (Shawn) Hsu

University of California, Irvine The Integrated Micro/Nano Summer Undergraduate Research Experience (IM-SURE) Single-Cell Platforms for Microbiomechanics Minh Guong Nguyen Biomedical Engineering University of California, Irvine. Mentor : Professor William C. Tang

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Mentor : Professor William C. Tang Grad Student : Yu-Hsiang (Shawn) Hsu

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  1. University of California, IrvineThe Integrated Micro/Nano Summer Undergraduate Research Experience (IM-SURE)Single-Cell Platforms for MicrobiomechanicsMinh Guong NguyenBiomedical EngineeringUniversity of California, Irvine Mentor: Professor William C. Tang Grad Student: Yu-Hsiang (Shawn) Hsu

  2. OUTLINE • Background • cytoskeleton • purposes • Introduction • QCM • our piezoelectric transducer • My responsibilities • design and develop experiments • collect and analyze resulting data • Problems and future work

  3. Intermediate filaments Microtubules Actin filaments protect cells and tissues from disintegration by mechanical stress essential component of cell division responsible for cell migration Fig. 1: Three types of protein filaments form the cytoskeleton CYTOSKELETON COMPONENTS Intermediate filaments Alberts, Bruce, et al. Essential Cell Biology. 2nd ed. New York & London: Garland Science, 2004

  4. Fig. 2: Forces generated move a cell forward ACTIN FILAMENTS Alberts, Bruce, et al. Essential Cell Biology. 2nd ed. New York & London: Garland Science, 2004

  5. PURPOSE mechanical changes of the cytoskeleton parallel drug screening cancerous cells identification and qualification others WHY SINGLE-CELL PLATFORMS ?

  6. cell chamber Fig 3: Sketch of the quartz crystal microbalance (QCM) experimental setup Fig. 4: A Single Cell Platforms for Microbiomechanics COMPARISON • Cannot detect 1 single cell mechanical • property • Not a precise result • Detect 1 single cell mechanical • property • A precise result Jing Li, Christiane Thielemann, Ute Reuning, and Diethelm Johannsmann. “Monitoring of integrin-mediated adhesion of human ovarian cancer cells to model protein surfaces by quartz crystal resonators: evaluation in the impedance analysis mode.” BioSensors & BioElectronics 20 (2005): 1333-1340. Online posting. http://www.wctgroup.eng.uci.edu/

  7. EXPERIMENTAL SETUP The Agilent 4395A The probe Picture is taken by Minh Guong Nguyen, BME student, UCI

  8. cell Au Au Si 200 µm CROSS SECTION OF OUR DEVICE ZnO SiO2 Cross section of our device, drawing by Yu-Hsiang (Shawn) Hsu, Ph.D candidate, Dept of BME, UCI

  9. 200 µm in Diameter (our device) Units in mm 1 mm square top electrode 15 µm thin lines TOP VIEW OF OUR DEVICE Top view of our device, drawing by Yu-Hsiang (Shawn) Hsu, Ph.D candidate, Dept of BME, UCI

  10. Anti-resonance frequency Resonance frequency Resonance frequency Impedance (Ω) Frequency (MHz) Fig. 6: The graphs Impedance vs. Frequency of our device OUR DEVICE’S IMPEDANCES Impedance vs. Frequency Anti-resonance frequency Data is collected by our experiments

  11. THE QUALITY FACTOR • QM: The quality factor • fa: The anti-resonance frequency (MHz) • fr: The resonance frequency (MHz) • Zr: The impedance at resonance frequency (Ω) • C: The static capacitance (pF) http://www.morganelectroceramics.com/tutorials/piezoguide15.html

  12. TABLE OF VALUES OF OUR DEVICES Data is collected by our experiments

  13. THE QUALITY FACTORS (QM) OF OUR DEVICES The average is 7.3585 Calculation is based on our data obtained from experiments

  14. COMPARISION OF OUR DEVICE WHEN TREATED WITH AND WITHOUTWATER Impedance vs. Frequency Without Water With Water Impedance (Ω) Frequency (MHz) Fig. 8: Comparison of our device when treated with and without water The graph is based on our data collected form experiments

  15. DATA ANALYSIS The frequency shift is related to the weight of water. The quality factor is related to the viscosity of water.

  16. Impedance vs. Frequency Impedance vs. frequency Impedance (Ω) Impedance (Ω) Fig. 10: Graph of impedance vs. frequency Fig. 11: Graph of impedance vs. frequency PROBLEMS AND FUTURE WORK GOOD Noise interferes the signal BAD Frequency (MHz)

  17. ACKNOWLEDGEMENTS • Dr. William C. Tang • Yu-Hsiang Hsu and John Lin • Wyman Wong • Said M. Shokair • Edward M. Olano • Sarah R. Martin • UROP Fellows • National Science Foundation ALL FOR YOUR SUPPORT

  18. QUESTIONS?

  19. Comparison of our device when treated with and without water Back up slide

  20. Impedance vs. Frequency Impedance of Resistor: ZR = R Impedance of Inductor: ZL = j ω L Impedance (Ω) Impedance of Capacitor: Zc = ω = 2 () (f) The graphs of impedance vs. frequency of our devices zoom-in Frequency (MHz) Back-up slide

  21. Inductor Capacitor Resistor Capacitor Fig 6: The BVD equivalent circuit Fig. 7: Lumped-element equivalent circuit Butterworth-Van-Dyke (BVD) equivalent circuit • Joachim Wegener, Jochen Seebach, Andreas Janshoff, and Hans-Joachim Galla. « Analysis of the Composite Response of Shear Wave Resonators to the Attachment of Mammalian Cells.» Biophysical Journal. Volume 78. June 2000: 2821-2833.

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