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Xu, Y.; Hoshi, Y.; Ober, C.K., J. Mater. Chem. , 2011 , in press, DOI: 10.1039/c0xx00000x.

Photo-Switchable Polyelectrolyte Brushes for Dual Protein Patterning Christopher K. Ober, Cornell University, DMR 1105253.

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Xu, Y.; Hoshi, Y.; Ober, C.K., J. Mater. Chem. , 2011 , in press, DOI: 10.1039/c0xx00000x.

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  1. Photo-Switchable Polyelectrolyte Brushes for Dual Protein Patterning Christopher K. Ober, Cornell University, DMR 1105253 Patterned protein arrays on substrates can greatly simplify the analysis of protein-protein or protein-cell interactions. When multi-component protein patterning is involved, more care is necessary to avoid cross-contamination and denaturation. We have created a photo-switchable polyelectrolyte brush with an o-nitrobenzyl-containing photo-reactive unit in which the surface charges can be changed from cationic to anionic by UV illumination at a wavelength of 365 nm. Surfaces can thus be patterned to produce square arrays and more complex configurations of cationic/anionic binary brushes. More importantly, dual protein patterns can be easily prepared by electrostatic attractions between the surface and the proteins as shown in the figure to the right. Fluorescence microscope images of patterned 9-aminoacridine (a) and fluorescein (b), a dual color fluorescence microscope image of patterned BSA-FITC and AV-TR (c) and patterned BSA and avidin detected with an orange fluorescent anti-BSA antibody and a green fluorescent biotin on the photo-responsive polymer brush (d), and intensity profiles on the dotted line (e, f). The scale bars represent 50 m. Xu, Y.; Hoshi, Y.; Ober, C.K., J. Mater. Chem., 2011, in press, DOI: 10.1039/c0xx00000x.

  2. Photo-Switchable Polyelectrolyte Brushes for Dual Protein Patterning Christopher K. Ober, Cornell University, DMR 1105253 • Ober has created and maintains the IUPAC Polymer Education website – it is one of the Google, Bing and Yahoo top hits for this topic • This site is designed to be a resource for students and teachers • Ober leads efforts by the IUPAC Polymer Division to celebrate 2011, the International Year of Chemistry (IYC) • IUPAC has sponsored a writing and video contest on “A World without Polymers”. Winning entries can be found at: http://www.iupac.org/polyedu/page42/styled-14/index.html • Other outreach activities include hosting REU students, international student exchanges, involvement in teacher workshops and visits to local schools Award winner of the video competition, Yvonne Lann, a high school student from Malaysia. Shown at the World Chemical Congress in Puerto Rico, before receiving her award with her parents, Prof. Jung-Il Jin, past president of IUPAC and Chris Ober, president of the IUPAC Polymer Division.

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