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Lesson #11: Enzymes in digestion

Lesson #11: Enzymes in digestion. Objective: - Identify the main enzymes in digestion and their functions. Practical. Label the boiling tubes A & B Fill each tubing with 8ml of starch . Wash one of the tubes very well and place it in boiling tube A.

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Lesson #11: Enzymes in digestion

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  1. Lesson #11: Enzymes in digestion Objective: - Identify the main enzymes in digestion and their functions

  2. Practical • Label the boiling tubes A & B • Fill each tubing with 8ml of starch. • Wash one of the tubes very well and place it in boiling tube A. • Add 3ml of amylase solution in the other tube. Shake. Wash very well and place in boiling tube B. • Place both boiling tubes in the water bath for 30 minutes. • Test the water in the boiling tube for starch and glucose using iodine and benedict’s solution • Record your results.

  3. Digestive Enzymes • There are 3 main types of digestive enzymes:

  4. Amylase Protease Digestive Enzymes Glucose Starch Amino Acids Protein

  5. Digestive Enzymes Lipase Fatty Acid Glycerol Fatty Acid Glycerol Fatty Acid Fatty Acid Fatty Acid Fatty Acid Fatty Acids and Glycerol Fat

  6. The enzyme fits over the substrate perfectly – like a key fits a lock. Amylase enzyme Starch molecule It holds the starch molecule in place as a water molecule breaks the bond between two glucose particles

  7. This continues until the molecule has been broken down completely

  8. Digestion Summary Chemical digestion: • Salivary amylase: starch  maltose • Pepsin in stomach: protein  peptides • Pancreatic amylase: starch  maltose • Trypsin from the pancreas: protein  peptides • Lipase from the pancreas: fats  glycerol + fatty acids

  9. What factors affect the rate of reaction of enzymes • How do you think you can speed up the rate of an enzymic reaction?(Hint: It’s the same as any other reaction!)

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