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Sample 1 – Cy3. Sample 2 – Cy5. Overlay Image.
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Sample 1 – Cy3 Sample 2 – Cy5 Overlay Image Samples are labelled with different fluorescent tags, allowing 2 samples to be separated on a single gel, removing gel-to-gel variation. An internal standard, composed of all samples in the experiment, is run on each gel, allowing comparison of samples on different gels. DIGE comparison of depleted plasma