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Life Cycles Laboratory

Life Cycles Laboratory. Observations of Life Cycle Stages in Protists and Plants. Haplontic Life Cycle of Chlamydomonas. Resting spore withstands poor conditions. Photosynthetic protist showing an early eukaryotic life cycle No mitosis in diploid phase.

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Life Cycles Laboratory

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  1. Life Cycles Laboratory Observations of Life Cycle Stages in Protists and Plants

  2. Haplontic Life Cycle of Chlamydomonas Resting spore withstands poor conditions • Photosynthetic protist showing an early eukaryotic life cycle • No mitosis in diploid phase To observe: Place a drop from the cell culture between a slide and cover slip.

  3. Isogametes fuse to form diploid zygote Gametangium: cell that produces gametes by mitosis Haplontic Life Cycle of Ulothrix • Photosynthetic Protist • No mitosis in diploid phase • Zygote produces spores by meiosis • Haploid gametophyte grows from spore by mitosis • Gametophyte can produce gametes or zoospores • Motile spores (zoospores) formed by mitosis or meiosis Sporangium: cell that produces spores by mitosis Spore: haploid cell that can grow by mitosis Gametophyte: multicellular haploid structure that produces gametes

  4. Haplontic Life Cycle of Ulothrix To observe: Place a drop from the cell culture between a slide and cover slip.Include some green fibers in your sample. You do not need to distinguish between gametangia and sporangia.

  5. Diplohaplontic Life Cycle of Ulva • Photosynthetic Protist • Introduction of Mitosis in Diploid phase • Gametophyte produces isogametes • Isomorphic gametophyte and sporophyte Sporophyte: multicellular diploid structure that produces spores by meiosis

  6. Diplohaplontic Life Cycle of Ulva To observe: Use a scalpel and/or forceps to take a small sample of the leafy structure and place it between a slide and cover slip. You do not need to distinguish between sporophytes and gametophytes.

  7. Archegonium: organ that produces eggs by mitosis Diplontic Life Cycle of Fern • Plant • Smaller gametophyte • Larger, longer-lived sporophyte with tissues and organs • Spores producedby meiosis in sporangia on the underside of sporophyte leaves Antheridium: organ that produces sperm by mitosis contain sporangia(singular: sorus)

  8. Prepared Slide: Mature Antheridia Sperm within Antheridia Gametophyte with Antheridia

  9. Prepared Slide: Mature Archegonia Each archegoniumcontains one egg Gametophyte with Archegonia

  10. Sporling Gametophyte Prepared Slide: Young Sporling

  11. Spores Indusium Sporangium Observation: Sorus, Sprorangia and Spores To observe: Scrape some material from the underside of a fern leaf onto a slide. Distribute it into a thin layer. Add a cover slip. Adding water is optional.

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