Team Pegasus MSF SatElysium Critical Design Review

1. Team Pegasus MSFSatElysiumCritical Design Review Jordan Burns, Brenden Hogan, Miranda Link, Cody Spiker, Chris Dehoyos, HemalSemwal October 4th, 2011

2. Mission Overview • Mission Statement: • “To test the effects of high altitude flight upon a closed contatiner containing bacterium and recording their response to discover if harsh high altitude effects bacterial reproduction and survival.” • Objective: • Test and record the effects of a near space environment on several cultures of Streptococcus mutans.  • Purpose: • Determine how bacteria cultures respond to the lack of pressure, extreme temperatures, and radiation present in near-space. • Why: • Test the validity the of the recorded results of the 1967 flight of Surveyor III. According to their results, we think that we will find that the bacteria not only survive, but actually thrive in the harsh environment of space. 

3. Requirements Flow-Down Level 0

4. Requirements Flow-Down Level 1

5. Design • Design • 21 cm cube • 2 levels • Bacteria - 6 different samples, 3 locations with varying conditions • Radiation • Temperature • Heated and isolated • Ground control (4) samples • Experiment structure • Petri-dish orientation – tilted for space • Motorized door to expose one sample to the outside environment • Petri-dish support structure • Analysis of Bacteria (before and after flight) • Colony Count (avg.) • Color • Spores

6. Design • Microcontroller • Pressure • Temperature • Light • Hobo Sensors • Heater (2) • For technical components and (1) bacteria sample

7. Functional Block Diagrams

8. Design 9 volt Batteries Aluminum Tape Humidity & Temp. Sensor Styrofoam Live Strand of Streptococcus Mutans AGAR Motor Light Sensor- NOT ORDERED Pressure Sensor – NOT ORDERED Parts: Solder Foam Core HOBO ArduinoPro Digital Camera Heating System Divided Petri Dishes

9. Schedule • 9/12/11: Divide tasks and submit individual sections by 9/13/11 • 9/14/11: Team meeting/Take ITLL Tour to get access • 9/15/11: Finalize Proposal • 9/16/11: Submit Proposal • 9/19/11: Team meeting for Design Presentation • 9/20/11: Conceptual Design Review Presentation • 9/22/11: Team meeting to decide parts order forms. • 9/27/11: Order satellite hardware • 9/28/11-10/7/11: Build and test prototypes. Grow first set of bacteria for ground control. • 10/8/11-10/13/11: Complete testing and design modifications. • 10/16/11-10/23/11: Construct BalloonSat. • 10/23/11: Satellite completion • 10/25/11: Pre-Launch Inspection • 10/27/11: In class mission simulation • 11/01/11: Launch readiness review • 11/04/11: Final BalloonSat Weigh in and turn in • 11/05/11: Launch and Recovery • 11/06/11: Meet to review data • 11/14/11: Review final report • 11/21/11: Complete final report • 11/29/11: Final Team Presentations and Report • 12/03/11: Design Expo

10. Test Plan • Our testing will occur in two separate phases. • The first of these phases will take place during the weekend of October 7-9. • Structural integrity tests • Whip test • Drop test • Roll test • The second set of tests will occur after we have all of our materials gathered and ready to perform the necessary experiments. • The Incubation and Vacuum Chamber • The Cooler test will be the last thing • need the entire completed BalloonSat ready to run a full-time data collection trial with all components of the working spacecraft in order. • The software/hardware testing will occur repeatedly throughout our entire building process as things will need to be adjusted every so often.

11. Testing • Phase I: Structural Testing • Drop/ Roll Test • Roll down flight of stairs • Drop from a height of 15 meters • Whip Test • Spinning the Balloonsat by the tether running through it. • Making sure that the balloonsat can withstand the forces acting upon it during the flight environment. Different starting orientations to ensure proper testing

12. Testing cont’d • Phase II: Scientific Materials Testing • Incubation Test • Temperature, light, and pressure controlled environment to have a sample. • Grown bacteria cultures throughout the semester to analyze a “natural” growth pattern. • Vacuum Chamber Test • Hand in Hand with the incubation test, merely used as a ground test to show the ground effects of the bacteria with a “zero pressure” environment • Software / Hardware Testing • Recursive tests involving the wiring and technical setup of the camera and temperature sensor arrangement to allow for different capturing intervals of the camera and data collections by the individual sensors that we have. • Cooler Test • Final test of our satellite. Will ensure that all the technical components will last for the duration of an entire flight sequence at temperatures exceeding -70­°F.

13. Expected Results • We expect to observe bacteria response, • bacterium count, health, reproduction, death and other anomalies • Compare resulting bacterium tests of both the baseline as well as the space bacteria. • use a microscope • Specifically look at spore count • We expect to find that the bacteria will be resilient enough to survive in the harsh environment. • Since our satellite will have three separate environments for testing, there is a real chance of seeing a change between each environment.

14. Budget

15. Team Organization

16. Worries • That our petri-dishes may not properly fit in SatElysium. • That our insulation system will not properly separate the separate sections into their individual environments that need to be attained. • Attaining the proper structural integrity that will allow us to successfully have two separate levels in our SatElysium.