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Table 2. Set of reference peptide for retention time correction. *HF refers to the hydrophobicity factor calculated using the SSRCalc algorithm [1, 2]. [1] Krokhin, O. V., Sequence-specific retention calculator. Algorithm for peptide retention prediction in
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Table 2. Set of reference peptide for retention time correction *HF refers to the hydrophobicity factor calculated using the SSRCalc algorithm [1, 2]. [1] Krokhin, O. V., Sequence-specific retention calculator. Algorithm for peptide retention prediction in ion-pair RP-HPLC: application to 300- and 100-A pore size C18 sorbents. Anal Chem 787785-7795 (2006). [2] Spicer, V., Yamchuk, A., Cortens, J., Sousa, S., et al., Sequence-specific retention calculator. A family of peptide retention time prediction algorithms in reversed-phase HPLC: applicability to various chromatographic conditions and columns. Anal Chem 79 8762-8768 (2007).