1 / 21

Genomics II: The Proteome

Genomics II: The Proteome. Using high-throughput methods to identify proteins and to understand their function. Protein Complementation. Enzymatic complementation b -galactosidase reconstitution Fluorescence complementation GFP or YFP reconstitution

vail
Download Presentation

Genomics II: The Proteome

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Genomics II:The Proteome Using high-throughput methods to identify proteins and to understand their function

  2. Protein Complementation • Enzymatic complementation • b-galactosidase reconstitution • Fluorescence complementation • GFP or YFP reconstitution • FRET (fluorescence resonance energy transfer)

  3. Enzymatic Complementation

  4. Blue=DAPI Red=BGAL Blue=DAPI Green=BGAL Red=Actin

  5. Bimolecular Fluorescence Complementation

  6. FRET (fluorescence resonance energy transfer)

  7. Summary I • Goals of proteomics • Identify and ascribe function to proteins under all biologically plausible conditions • Proteomics methods • 2-D gel electrophoresis for separating proteins on the basis of charge and molecular weight • Mass spectrometry for identifying proteins by measuring the mass-to-charge ratio of their ionized peptide fragments • Protein chips to identify proteins, to detect protein–protein interactions, to perform biochemical assays, and to study drug–target interactions

  8. Summary II • Proteomics methods (continued) • Yeast two-hybrid method for studying protein–protein interactions • Biochemical genomics for high-throughput assays • Some accomplishments of proteomics • Example: yeast • Yeast two-hybrid method reveals interactome • Transcriptional regulatory networks deduced • Biochemical genomics uncovers new ORF functions • Subcellular localization of proteins

  9. Linkage and Mapping

  10. Linked alleles tend to be inherited together

  11. Crossing over produces new allelic combinations

More Related