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ATP dependence. Ca 2+ dependence. CaM 1234. CaM Pep G Pep H Pep I Pep J. CaM Pep G Pep H Pep I Pep J. CaM Pep G Pep H Pep I Pep J. CaM Pep G Pep H Pep I Pep J. P62 P63 P64 P65 CaM. P62 P63 P64 P65 CaM. 200 M free Ca 2+ CaM:peptide 1:8. 1 mM EGTA
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ATP dependence Ca2+ dependence CaM1234
CaM Pep G Pep H Pep I Pep J CaM Pep G Pep H Pep I Pep J CaM Pep G Pep H Pep I Pep J CaM Pep G Pep H Pep I Pep J P62 P63 P64 P65 CaM P62 P63 P64 P65 CaM 200 M free Ca2+ CaM:peptide 1:8 1 mM EGTA CaM:peptide 1:8 1 mM EGTA CaM:peptide 1:8 1 mM EGTA CaM:peptide 1:8 P62 P63 P64 P65 CaM1234 P62 P63 P64 P65 CaM1234 200 M free Ca2+ CaM1234:peptide 1:8 1 mM EGTA CaM1234:peptide 1:8
EGTA Ca2+ Suramin Suramin CaM CaM CaM CaM 50 µM 50 µM 0 µM 25 µM 100 µM 0 µM 25 µM 100 µM 50 µM 50 µM 0 µM 25 µM 100 µM 0 µM 25 µM 100 µM P13 P14 P14 P13