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Spooling DNA

Spooling DNA. Source: Strawberries Octoploid (8 copies of their genomic DNA). DNA Extraction . Place strawberry in baggie and close baggie “mush” berry for about 2 min. Add DNA extraction buffer Close baggie, and “mush” berry again for 2 min. DNA Extraction.

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Spooling DNA

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  1. Spooling DNA Source: Strawberries Octoploid (8 copies of their genomic DNA)

  2. DNA Extraction • Place strawberry in baggie and close baggie • “mush” berry for about 2 min. • Add DNA extraction buffer • Close baggie, and “mush” berry again for 2 min.

  3. DNA Extraction • Set-up beaker with cheese cloth • Cut corner of baggie and pour strawberry solution into cheese cloth • Squeeze out solution into beaker, the waste/pulp is trapped in cheese cloth • The strawberry solution contains your DNA

  4. DNA Extraction • Fill the test tube 1/3rd to ½ with the strawberry solution. • Now very slowly using the pipet add ice cold ethanol down the side of the test tube. Add enough until the tube is about 3/4th filled and you should see a whitish material. You want two layers to form. • Now take the wooden stick and begin “spooling”. Place the stick in near the interface of ethanol and the strawberry solution and “twirl”. • DO NOT MIX ETHANOL WITH THE STRAWBERRY SOLUTION.

  5. You have now isolated genomic DNA from strawberries!

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