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In the name of God

In the name of God . Summer School. Influenza Unit, Pasteur Institute of Iran summer 2010. Laboratory Animals Experimentation. A.Torabi A.Khajevand. Laboratory Animal Experimentation. Rabbit. Summer School .

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In the name of God

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  1. In the name of God

  2. Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  3. Laboratory Animals Experimentation A.Torabi A.Khajevand

  4. Laboratory Animal Experimentation Rabbit Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  5. Laboratory Animal Experimentation- Rabbit • A. Intramuscular injection • B. Subcutaneous injection • C. Blood collection from ear vein • D. Blood collection from carotid artery • E. Sexing Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  6. Intramuscular Injection in Rabbit • The most common site for administer antibiotic, tranquilizer and anesthetic Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  7. Tools for intramuscular injection in rabbit Summer School • Hypnovel and Hypnorm for general anesthesia. • 75% alcohol cotton ball for surface disinfection • 27G needle with 1 ml syringe • 24Gneedle with 5ml syringe Influenza Unit, Pasteur Institute of Iran summer 2010

  8. Summer School Disinfect of skin on the top of thigh muscle Influenza Unit, Pasteur Institute of Iran summer 2010

  9. Summer School Insert needle perpendicular to the surface of skin and inject the substance inside the syringe Influenza Unit, Pasteur Institute of Iran summer 2010

  10. Subcutaneous Injection in Rabbit • The most common method for immunology study Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  11. Tools for subcutaneous injection in rabbit Summer School • 75% alcohol cotton ball for surface disinfection • 21 G needle with 1 ml syringe for injection Influenza Unit, Pasteur Institute of Iran summer 2010

  12. Summer School Disinfect of injection site by 75% alcohol directly or 75% alcohol cotton ball Influenza Unit, Pasteur Institute of Iran summer 2010

  13. Summer School Pinch the skin up and insert the needle in for injection Influenza Unit, Pasteur Institute of Iran summer 2010

  14. Summer School Keep the needle tip in skin for a while after injection to prevent leakage of substance from the needle which due to imbalance pressure of emulsion inside the syringe. Influenza Unit, Pasteur Institute of Iran summer 2010

  15. Collection of Blood from Ear Vein in Rabbit • A common location for small amount of blood collection • May use tranquilizer for nervous rabbit or small size rabbit Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  16. Tools for blood collection from ear vein Summer School • 75% alcohol cotton ball for surface disinfection • 27G needle with 1 ml syringe for apply of tranquilizer • 24G needle with 5 ml syringe for blood withdraw • 15 ml centrifuge tube for blood collection • a plastic clip and a cotton ball for prevention of further bleeding Influenza Unit, Pasteur Institute of Iran summer 2010

  17. Summer School Ear vein before administering of tranquilizer Influenza Unit, Pasteur Institute of Iran summer 2010

  18. Summer School Ear vein after administering of tranquilizer Influenza Unit, Pasteur Institute of Iran summer 2010

  19. Summer School Insert needle into the ear vein with needle tip surface face up Influenza Unit, Pasteur Institute of Iran summer 2010

  20. Summer School Fix the position of needle by your left thumb and withdraw blood from the ear vein Influenza Unit, Pasteur Institute of Iran summer 2010

  21. Summer School Press a cotton ball on the top of the injection site before pull out the needle. Influenza Unit, Pasteur Institute of Iran summer 2010

  22. Summer School Apply clip on the top of the cotton ball for 2-3 minutes to stop further bleeding Influenza Unit, Pasteur Institute of Iran summer 2010

  23. Whole Blood Collection from Carotid Artery in Rabbit • Must under general anesthetic • May collect blood amount up to 4 % of body weight Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  24. Tools for collecting 50ml blood from ear artery Summer School • 75% alcohol cotton ball for disinfect of ear surface • 21 G winged infusion set with 50 ml syringe for blood collection • Dry cotton ball and plastic clip for prevention of further bleeding Influenza Unit, Pasteur Institute of Iran summer 2010

  25. Summer School Apply same procedure as collecting blood from ear vein for blood collection from ear artery Influenza Unit, Pasteur Institute of Iran summer 2010

  26. Tools for blood collection from carotid artery • Electric clipper • Vacuum cleaner • Scalpel blades and handle • Tissue forceps x2 • Scissors • Bulldog clamp • Homeostatic forceps • 3 - 000 silk suture • 16G Intravenous catheter • 50 ml Centrifuge tubes Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  27. Summer School Restrain the anesthetized rabbit on the surgical table Influenza Unit, Pasteur Institute of Iran summer 2010

  28. Summer School Remove the hair around the neck area Influenza Unit, Pasteur Institute of Iran summer 2010

  29. Summer School Make a 5 cm opening on the middle line of the neck Influenza Unit, Pasteur Institute of Iran summer 2010

  30. Summer School Search the carotid artery with a tissue forceps and a homeostatic forceps. Influenza Unit, Pasteur Institute of Iran summer 2010

  31. Summer School Isolate the carotid artery from the vagus nerve and other connective tissue Influenza Unit, Pasteur Institute of Iran summer 2010

  32. Summer School Tie up the distal end of the carotid artery by silk suture Influenza Unit, Pasteur Institute of Iran summer 2010

  33. Summer School Clip the proximal end of the carotid artery with a bulldog clamp Influenza Unit, Pasteur Institute of Iran summer 2010

  34. Summer School Insert a 16 G intravenous catheter into the artery Influenza Unit, Pasteur Institute of Iran summer 2010

  35. Summer School Fix the position of the catheter tip by two silk suture knots Influenza Unit, Pasteur Institute of Iran summer 2010

  36. Summer School Release the bulldog clamp and collect blood from the artery though the catheter into the centrifuge tube Influenza Unit, Pasteur Institute of Iran summer 2010

  37. Slight pressure is applied to the external genitalia to expose the vulva of a female bunny Summer School Influenza Unit, Pasteur Institute of Iran summer 2010

  38. Summer School A female rabbit Influenza Unit, Pasteur Institute of Iran summer 2010

  39. Summer School A male rabbit Influenza Unit, Pasteur Institute of Iran summer 2010

  40. Aquire Specialty to Take Opportunity Thanks

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