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Gene Discovery Research in Grapevine and Applications to Viticulture

UBC Wine Research Centre ? Growing by Leaps and Bounds. Founded in 1999 by Dr. Hennie van Vuuren, WRC Director ? 7 members2002 HJVV obtained Western Diversification funding to renovate five former Home Economics labs into three state-of-the-art molecular biology labs for faculty and two core facil

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Gene Discovery Research in Grapevine and Applications to Viticulture

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    1. Gene Discovery Research in Grapevine and Applications to Viticulture

    2. UBC Wine Research Centre – Growing by Leaps and Bounds Founded in 1999 by Dr. Hennie van Vuuren, WRC Director – 7 members 2002 HJVV obtained Western Diversification funding to renovate five former Home Economics labs into three state-of-the-art molecular biology labs for faculty and two core facilities 2002 Wine Library opens, funded in part with donations from BC wineries 2002 Viticulture/Plant Genomics junior chair position filled by SL 2003 Yeast Genomics junior chair position filled by Dr. Vivien Measday Today, WRC consists of 3 faculty, 3 associate faculty, 17 staff and students – target is 30-35 total in Vancouver

    3. Potential for Future Expansion to UBC Okanagan Research at the WRC is focused on molecular biology and genomics of grapevine and wine yeasts Future expansion should include: Grapevine physiologists and geneticists Enologists Highly trained extension specialists to support viticulturalists and wine makers throughout BC

    4. Viticulture Genomics Research Program at UBC (Lund lab) Control of ripening initiation and metabolism in Cabernet Sauvignon berries (currently funded by Genome Canada) Mechanisms underlying freezing tolerance in deacclimating buds in Cabernet Sauvignon and Vitis riparia cv. Gloire (funding to be secured)

    5. GrapeGen: A Genomic Approach to the Identification of the Genetic and Environmental Components Underlying Berry Quality in Grapevine ($6.2 million Cdn, 3 yr.)

    6. GrapeGen Goals Determine the molecular basis for ripening initiation and the metabolism of compounds important for grape must and wine quality Determine how gene expression and metabolite profiles are affected by different environmental conditions (diffuse sunlight versus heavy canopy shading) Use sequence and expression profiling data to identify key DNA markers for: 1) diagnostic tool development and 2) molecular breeding and clonal selection efforts

    8. Berry Growth and Development

    11. Cellular Signaling for Ripening Initiation

    12. Does a Vacuole Transporter Control Acidity in Grape Cells?

    13. Enzymes Control the Nuances of Flavour and Aroma Through the Production of Metabolites (collaboration with Dr. Joerg Bohlmann, UBC)

    14. Non-GMO Applications of Grape Genomics Research Data Genomics GMOs DNA sequences can be printed onto chips (called ‘microarrays’). These could be used to monitor berry development and vine health throughout the growing season: Predict timing of veraison with accuracy each season Assess drought, cold, pathogen stresses Monitor and forecast metabolite profiles Monitor response in berries to C:N ESTs can be mapped to genetic loci important for berry quality. This information can be used in breeding programs to exploit natural genetic variation in Vitis relatives.

    15. GM Grapevines? What the Future Could Bring GM involves the introduction of up to 5 thousand DNA base pairs into the grapevine diploid genome of 950 million base pairs (that’s a 0.0005% change!) Potential applications of GM to berry production: Accelerate ripening initiation and shorten the season Control acid content/pH Tailor metabolite profiles and improve balance at harvest Generate smaller, darker berries

    17. GrapeGen Project Team - Canada

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