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SUBMITTED BY :- GAURAV KUMAR PAL

TOPIC :- EVALUATION OF ANTIMICROBIAL EFFECTIVENESS & FUNCTION OF LAF. SUBMITTED BY :- GAURAV KUMAR PAL

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SUBMITTED BY :- GAURAV KUMAR PAL

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  1. TOPIC :- EVALUATION OF ANTIMICROBIAL EFFECTIVENESS & FUNCTION OF LAF SUBMITTED BY :- GAURAV KUMAR PAL M.Sc.Applied Microbiology

  2. Content :- • INTRODUCTION • HISTORY • METHOD FOR TESTING DISINFECTANTS • SUSPENSION TEST • PHENOL COEFFICIENT TEST • METHOD FOR DETERMINATION OF PHENOL COEFFICIENT TEST • CARRIER TEST • IN USE TEST • PRACTICAL TEST • LAMINAR AIR FLOW • FUNCTION OF LAF

  3. INTRODUCTION:- • Testing of disinfectant is a complex process. • are used to test the efficacy of disinfectant. • Requlated by two different federal agencies- • U.S.environment protection assay-regulate to disinfectants. • Food &drug administration –regulated to disinfectants used on human & animals . • Disinfectants used mainly in hospital & labs, so periodally effectiveness of disinfectant is checked.

  4. HISTORY :- • Robert Koch described a disinfectant test in 1881. • Used a liquid culture of Bacillus anthracisfor testing of disinfectant. • A silk thread contaminated in liquid culture & drying • Immersed in several disinfectants & cultured in nutrient broth &incubated . 1.Growth occur – less effective disinfectants . 2. No Growth occur – more effective disinfectants .

  5. METHOD FOR TESTING DISINFECTANTS :- • Suspension tests • Carrier tests • In –use test • Practical test

  6. SUSPENSION TEST :- • A sample of bacterial culture is suspended into the disinfectant solution. • After exposure , it is verified by subculture of bacterial culture . • After subculture :- 1.Growth occur – less effective disinfectants . 2. No Growth occur – more effective disinfectants .

  7. PHENOL COEFFICIENT TEST :- • This method is used to evaluating the effectiveness of disinfectants . • Best known disinfectant test. • Staphylococcus aureusor Salmonella typhi are used for testing . • Dilution of phenol &experimental disinfectant are inoculated with Staphylococcus aureus &incubated at 20-37 *c for 2-3 days. • Samples are removed at 5 min intervals and inoculated into fresh broth. • Highest dilution that kills the bacteria after the 10 mintues, but not after 5 mintues,is used to calculate the phenol coefficient test.

  8. PHENOL COEFFICIENT TEST (CONT.) • Phenol coefficient is calculated by dividing the reciprocal dilution of test disinfectants by the reciprocal dilution of phenol. • For example :- • The reciprocal of max. effective dilution of phenol =100 & the reciprocal of max. effective dilution for disinfectant=600, then phenol coefficient =600/100= 6 • If the value of phenol coefficient is more then 1 then test disinfectant is more effective then phenol.

  9. Method for determination of phenol coefficient:- • Rideal walker method :- • In this method different dilution of phenol & disinfectant is used. • Phenol coefficient is calculated by this method Phenol coefficient =the dilution of disinfectant (which disinfects the suspension in a given time )/dilution of phenol in same time. • Disadvantage :- • No organic matter is included. • Time allowed for disinfectant is short.

  10. CHICK MARTIN METHOD :- • In this method water is used with yeast suspension to stimulate the organic matter . • Time of subculture is fixed at 30 mintues . • Phenol coefficient is calculated as fellows- • Phenol coefficient = mean conc. of phenol /mean conc. of test disinfectant .

  11. CARRIER TEST :- • USE DILUTION TEST :- • These test are the oldest test . • AOAC used dilution test is a carrier based test. • S.aureus bacteria culture is used. • Metal cylinder dipped into broth culture . • Cylinder washed & placed into tube of medium for 48 hours. • Used disinfectant for 10 mintues . 1.Growth occur – less effective disinfectants . 2. No Growth occur – more effective disinfectants

  12. In use test • Use disinfectant to injured cell . • Swab taken before and after use of disinfectant. • Inoculated in growth medium. • Incubated for 48 hours. • Growth occurred-less effective • No growth- more effective.

  13. Practical test • Filter paper method:- • Disc of filter paper are used . • Filter paper are soaked with a chemical disinfectant. • They are placed in agar plate and incubated. • If the chemical is effective then clear zone appears near the disc.

  14. FILTER PAPER METHOD :-

  15. Laminar air flow • Laminar air flow is an equipment which can produce air flow continuously with uniform velocity. • Containing special HEPA filter which can remove microorganism and particles as small as 0.3 micrometer. • They are containing U.V. light to kill the microbes. • These are two types….

  16. Horizontal Laminar Air Flow Hood

  17. Vertical Laminar Flow Hood

  18. Function of L.A.F • It provide the sterile environment for the culturing . • Transfer any media for culturing the fungi and bacteria. • Use to prevent from contamination during the process of pouring in plates. • Observation of microbes.

  19. Reference • Prescott & harley ,Klein (2002) ,Microbiology second edition , wm.C.brown publishers. • Tortora J.G., Funk R.b. ,Christine l. case, (1997),microbiology an introduction sixth edition ,Addison Wesley Longman • ALCAMO I. EDWARD(2001), Fundamentals of microbiology fifth edition ,Jones and Bartlett publishers • Wistreich A.G.,MAX. D.lechtman(1988) Microbiology fifth edition , Collier macmillan publishers LANDON • http://WWW.ncbi.nlm.nih.gov/pmc/articles • http://www.antimicrobialtestlaboratories.com • http://www.microrao.com/micronotes/pg/testing_for_disinfectants.pdf

  20. THANKS

  21. GAURAV KUMAR PAL M.Sc. APPLIED MICROBIOLOGY MOBILE NO- 08979889920 ROOM No. - 38

  22. ANUJ KUMAR SHARMA M.Sc. BIOTECHNOLOGY MOBILE NO- 09536313795 ROOM No. - 38

  23. BRIJESH KUMAR M.Sc. APPLIED MICROBIOLOGY MOBILE NO- 09758505565 ROOM No. - 79

  24. CHANGHAGE KHAN M.Sc. CHEMISTRY MOBILE No.-8057380695 ROOM No. - 79

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