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Cholesterol

Cholesterol. Daheeya AlEnazi. -Cholesterol is a fat-like substance that is found in all body cells. - The liver makes all of the cholesterol the body needs to form cell membranes and to make certain hormones. -Used for the synthesis of bile and vitamin D.

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Cholesterol

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  1. Cholesterol DaheeyaAlEnazi

  2. -Cholesterol is a fat-like substance that is found in all body cells. -The liver makes all of the cholesterol the body needs to form cell membranes and to make certain hormones. -Used for the synthesis of bile and vitamin D. -High blood cholesterolis one of the major risk factors for heart disease. -Major dietary sources of it cheese, egg yolks ,beef, fish, and shrimp

  3. Principle: The cholesterol present in the sample originates a colored complex, according to the following reaction: Cholesterol esters + H2O CHE Cholesterol + fatty acids Cholesterol + O2 CHOD 4-Cholestenona + H2O2 2H2O2 + Phenol + 4-Aminophenazone POD Quinonimine+4H2O The intensity of the color formed is proportional to the cholesterol concentration in the sample

  4. Samples: Serum or plasma Procedure: Wavelength…….505 nm Cuvette……………1 cm light path Temperature………37ºc Reference Values: Less than 200 mg/dL…………Normal 200-239 mg/dL……………….Borderline 240 mg/dL and above ………High

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