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ENHANCE MENT OF EFFICIENCY OF ETHANOL PRODUCTION FROM JERUSALEM ARTICHOKE. Quang D. Nguyen, Kálmán Dénes , Csilla Farkas , Ágoston Hoschke , Judit M. Rezessy-Szabó. T he 2 n d International Symposium „Vera Johanides “ 2013 .
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ENHANCEMENT OFEFFICIENCY OF ETHANOL PRODUCTION FROM JERUSALEM ARTICHOKE Quang D. Nguyen, KálmánDénes, CsillaFarkas, ÁgostonHoschke, Judit M. Rezessy-Szabó The 2ndInternational Symposium „Vera Johanides“ 2013
Production of bioethanol in EU-27 - 2009(millionliters) Total: 3703 WelcomeCroatiato be inthis map (EU-28)! On the 3rdInternational Symposium „Vera Johanides“ in 2015 – Biotechnology and Forestry Complex in Croatia? Source: www.biofuels-platform.ch
Potential of JA-based bioethanol production • Jerusalem artichoke is easy to cultivate • Relatively high production yields: 16–20 t/ha (even 20-80 t/ha) for tubers, and 18–28 t/ha green weight for foliage • High carbohydrate content: 15-20 % mainly inulin and fructo-oligosaccharides • Cultivate yearly • Can be harvested like potato
Problems addressed • Saccharomyces cerevisiae strain does not exhibit so high inulinase activity • Treatment: acidic or enzymatic • Exogenousenzymepreparation • Low efficiency of bioconversion (60-70 %)
Objectives Technological development for enhancementof efficiency of ethanol productionusingJerusalemartichokeasrawmaterial • Inoculationtechnique • Simultan saccharification and fermentation • Semi-continuousfermentationtechnology
Development of inoculationtechnique • Effects of variousinoculationtechniqueswereinvestigated Best one oninitialday 2/3 amount of inoculum (cellnumber) and onsecondday: 1/3 amount Efficiency of bioconversion: 90 %
SSF vs. Mixed CultureFermentation Efficiency of bioconverion: 85-88 %
Semi-continuoustechnology Fresh mixed culture Fresh mash Harvested
Conclusion The use of mixed culture incombinationwithsemi-continuousfermentationtechnologydefinetelyincreasesinthe productivity, thusit is verypromising for development of technology for production of bioethanol based on Jerusalem artichoke as raw material
Acknowledgement This work was supported by Hungarian Development Agency through Project number TÁMOP-4.2.1./B-09/1-KMR-2010-0005and TECH_09-A3-2009-0194, as well as Hungarian Academy of Science through BolyaiJános Research Grant