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SUPPLEMENT 2 This power point presentation reports analyses of RyR distribution

SUPPLEMENT 2 This power point presentation reports analyses of RyR distribution measured by confocal microscopy in tangential sections of 6 SA node cells. Summary of the analyses. * Cell numbers are those assigned for 25 cells reported in Supplemental Figure 1.

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SUPPLEMENT 2 This power point presentation reports analyses of RyR distribution

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  1. SUPPLEMENT 2 This power point presentation reports analyses of RyR distribution measured by confocal microscopy in tangential sections of 6 SA node cells Summary of the analyses * Cell numbers are those assigned for 25 cells reported in Supplemental Figure 1 ** The SD in finding peaks with higher amplitude (>mean+2 x SD) was calculated for fluorescence peaks given in the units of the SD of the total fluorescence signal

  2. cell#5_bottom original 5* 20 mm

  3. cell#5_bottom Intensity peaks of RyR labeling identified by “SD-slicing” computer algorithm (color dots). Peak amplitudes are coded by a rainbow-color scheme -0.6 0 0.6 1.2 1.8 2.4 3.0 3.6 4 4.6 5.2 5.8 Units are SD of total cell signal Mean of total cell signal

  4. cell#5_bottom Results All peaks Peaks with higher amplitudes (> mean + 2 x SD) Nearest neighbor distance Nearest neighbor distance # of peaks # of peaks mm mm Peak amplitude Peak amplitude # of peaks # of peaks SD SD

  5. cell#10 bottom original 10* 20 mm

  6. cell#10 bottom_part2 Intensity peaks of RyR labeling identified by “SD-slicing” computer algorithm (color dots). Peak amplitudes are coded by a rainbow-color scheme -0.6 0 0.6 1.2 1.8 2.4 3.0 3.6 4 4.6 5.2 5.8 Units are SD of total cell signal Mean of total cell signal

  7. cell#10 bottom_part2 Results All peaks Peaks with higher amplitudes (> mean + 2 x SD) Nearest neighbor distance Nearest neighbor distance # of peaks # of peaks mm mm Peak amplitude Peak amplitude # of peaks # of peaks SD SD

  8. cell#8_top original 8* 20 mm

  9. cell#8_top Intensity peaks of RyR labeling identified by “SD-slicing” computer algorithm (color dots). Peak amplitudes are coded by a rainbow-color scheme -0.6 0 0.6 1.2 1.8 2.4 3.0 3.6 4 4.6 5.2 5.8 Units are SD of total cell signal Mean of total cell signal

  10. cell#8_top Results All peaks Peaks with higher amplitudes (> mean + 2 x SD) Nearest neighbor distance Nearest neighbor distance # of peaks # of peaks mm mm Peak amplitude Peak amplitude # of peaks # of peaks SD SD

  11. cell#11_bottom (main text Fig.8) original 11* 20 mm

  12. cell#11_bottom (main text Fig.8) Intensity peaks of RyR labeling identified by “SD-slicing” computer algorithm (color dots). Peak amplitudes are coded by a rainbow-color scheme -0.6 0 0.6 1.2 1.8 2.4 3.0 3.6 4 4.6 5.2 5.8 Units are SD of total cell signal Mean of total cell signal

  13. cell#11_bottom (main text Fig.8) Results All peaks Peaks with higher amplitudes (> mean + 2 x SD) Nearest neighbor distance Nearest neighbor distance # of peaks mm mm Peak amplitude Peak amplitude # of peaks SD SD

  14. cell#11_top original 11* 20 mm

  15. cell#11_top Intensity peaks of RyR labeling identified by “SD-slicing” computer algorithm (color dots). original Peak amplitudes are coded by a rainbow-color scheme -0.6 0 0.6 1.2 1.8 2.4 3.0 3.6 4 4.6 5.2 5.8 Units are SD of total cell signal Mean of total cell signal

  16. cell#11_top Results All peaks Peaks with higher amplitudes (> mean + 2 x SD) Nearest neighbor distance Nearest neighbor distance # of peaks # of peaks mm mm Peak amplitude Peak amplitude # of peaks # of peaks SD SD

  17. cell#13_bottom original 13* 20 mm

  18. cell#13_bottom Intensity peaks of RyR labeling identified by “SD-slicing” computer algorithm (color dots). Peak amplitudes are coded by a rainbow-color scheme -0.6 0 0.6 1.2 1.8 2.4 3.0 3.6 4 4.6 5.2 5.8 Units are SD of total cell signal Mean of total cell signal

  19. cell#13_bottom Results All peaks Peaks with higher amplitudes (> mean + 2 x SD) Nearest neighbor distance Nearest neighbor distance # of peaks # of peaks mm mm Peak amplitude Peak amplitude # of peaks # of peaks SD SD

  20. original cell#19_bottom 19* 20 mm

  21. cell#19_bottom Intensity peaks of RyR labeling identified by “SD-slicing” computer algorithm (color dots). Peak amplitudes are coded by a rainbow-color scheme -0.6 0 0.6 1.2 1.8 2.4 3.0 3.6 4 4.6 5.2 5.8 Units are SD of total cell signal Mean of total cell signal

  22. cell#19_bottom Results All peaks Peaks with higher amplitudes (> mean + 2 x SD) Nearest neighbor distance Nearest neighbor distance # of peaks # of peaks mm mm Peak amplitude Peak amplitude # of peaks # of peaks SD SD

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