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A Loregian , E Papini , B Satin , H S. Marsden , T R. Hirst , and G Palu PNAS

Intranuclear delivery of antiviral peptide mediated by the B subunit of Escherichia coli heat-labile enterotoxin. A Loregian , E Papini , B Satin , H S. Marsden , T R. Hirst , and G Palu PNAS Vol.96 pp.5221-5226, April 1999

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A Loregian , E Papini , B Satin , H S. Marsden , T R. Hirst , and G Palu PNAS

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  1. Intranuclear delivery of antiviral peptide mediated by the B subunit of Escherichia coli heat-labile enterotoxin A Loregian , E Papini , B Satin , H S. Marsden , T R. Hirst , and G Palu PNAS Vol.96 pp.5221-5226, April 1999 Speaker: 劉育男 Date: November 29, 2000 Place: 生科一館117教室

  2. Introduction: Compartmentalization : Effective compartmentalization of drugs is a prerequisite for evoking desired biological activity EtxB: E. coli heat labile enterotoxin B subunit HSV 1 UL42 accessory protein & POL peptide

  3. A Subunit B Subunit

  4. DNA Polymerase POL UL42 accessory protein

  5. Inhibitory POL POL with DNA polymerase UL42 accessory protein

  6. POL peptide sequence

  7. In vitro effect of EtxB-POL chimera EtxB POL EtxB-POL

  8. Anti-EtxB, anti-POL and anti-ExtB/POL Labeling

  9. Early Endosome Trans Golgi Network Lysosome Late Endosome

  10. Anti-transferrin receptor for early endosome

  11. Anti V-ATPase for late endosome & lysosome Anti gamma-adaptin for trans-Golgi network

  12. Effect of different drugs on intranuclear localization of Pol Golgi disruption Inhibit microtubule Inhibit v-ATPase

  13. EtxB-POL EtxB POL Inhibition of plaque-forming

  14. Inhibition of virus yield

  15. Inhibition of viral DNA synthesis

  16. Conclusion : • Chimeras can be used as Compartmentalizing tools. • EtxB can deliver biological active macromolecule such as vaccines, adjuvants and drugs. • Mimic subunit interface function to disrupt protein-protein interaction in a highly specific manner.

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