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A Synthetic Biology Approach to Heavy Metal Detection

A Synthetic Biology Approach to Heavy Metal Detection. Eric Yang and Qiang Shi Emporia State University. Heavy Metal Poisoning. Arsenic, mercury, lead, antimone, cadmium, etc. Contamination of water and soil Oxidative stress to cells --- damage to DNA and proteins

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A Synthetic Biology Approach to Heavy Metal Detection

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  1. A Synthetic Biology Approach to Heavy Metal Detection Eric Yang and Qiang Shi Emporia State University

  2. Heavy Metal Poisoning • Arsenic, mercury, lead, antimone, cadmium, etc. • Contamination of water and soil • Oxidative stress to cells --- damage to DNA and proteins • Disrupt function in vital organs and glands • Example: Mercury Effects • adrenal dysfunction, kidney damage, allergy, alopecia, anorexia, anxiety, birth defects, blushing, brain damage, cataracts, cerebral palsy, poor coordination / jerky movements, deafness, depression, dermatitis, discouragement, dizziness, drowsiness, eczema, emotional disturbances, excess saliva, fatigue, gum bleeding and soreness, headaches (band type), hearing loss, hyperactivity, hypothyroidism, forgetfulness, immune dysfunction, insomnia, irritability, joint pain,, loss of self-control, memory loss, mental retardation, metallic taste, migraines, nervousness, nerve fiber degeneration, numbness, pain in limbs, rashes, retinitis, schizophrenia, shyness, speech disorders, suicidal tendencies, tingling, tremors (eyelids, lips, tongue, fingers, extremities), vision loss, weakness

  3. Goals of the Model Design • Simultaneous detection of mercuric and arsenic contamination • Accumulating and cleaning mercury and arsenic.

  4. Ars Operon

  5. Mercury Operon

  6. Cloning modules Transporting Module • GlpF, a aquaglycerol porin from E. coli • Citrate coupled transporters, CitM from Bacillus subtilis • Promoter + MerR + MerP + MerT

  7. Cloning modules Detecting Module • Arsenic promoter from E. coli, pArsR + RBS + GFP • Mercury-Inducible Promoter from E. coli, pMerR + RBS + RFP

  8. Cloning modules Sequestering Module • Metallothioneins • pArsR + RBS + GFP + pArsR + RBS + fMT (arsenic form) • pMerR + RBS + RFP + pMerR + RBS + MerR + MerP + MerT + fMT (mercuric form)

  9. Cloning modules Buoyancy Module • gas vesicle protein gene cluster from Bacillus megaterium, GVP • pArsR + RBS + GFP + pArsR + RBS + fMT (arsenic form) + RBS + GVP • pMerR + RBS + RFP + pMerR + RBS + MerR + MerP + MerT + fMT (mercuric form) + RBS + GVP

  10. Topological Sort

  11. Let’s Make a Deal

  12. Sudoku

  13. Acknowledgement • All organizers • All participants

  14. Thank you!

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