1 / 7

Exposure of zebrafish embryos to solvents. The statistical issues

Exposure of zebrafish embryos to solvents. The statistical issues. Current work. Exposure of Zebrafish embryos Various concentrations (0.002 – 4.0 % v/v) DMSO DMF Zebrafish embryo test 48hr exposure Observe embryos at 24 & 48 hrs. 1-2 hrs. 24hrs. 48hrs. Current Work II.

ward
Download Presentation

Exposure of zebrafish embryos to solvents. The statistical issues

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Exposure of zebrafish embryos to solvents.The statistical issues

  2. Current work • Exposure of Zebrafish embryos • Various concentrations (0.002 – 4.0 % v/v) • DMSO • DMF • Zebrafish embryo test • 48hr exposure • Observe embryos at 24 & 48 hrs 1-2 hrs 24hrs 48hrs

  3. Current Work II • Observe developmental abnormalities • Coagulation • Oedema • Lack of circulation • Reduced/No heart beat • Produce dose response curves • Determine toxicology statistics • NOEC’s • LDC’s • EC’s

  4. Current Work III Dye coupled samples • Gene expression changes • Microarrays Microarray representing ~16,000 Zebrafish genes Ratio of CY 5 & CY 3 fluorescence

  5. Statistical Issues II • Microarrays • 17,000+ pieces of data per array • Eliminate poor spots • Eliminate variation • Dye effect, probe length, background, technical • Normalise data • Determine significance of any up or down regulation • ANOVER • Statistical programs (Genespring, R)

  6. Statistical Issues III • Problems • Lack replicates • False positives • Use very small p values • Variation within arrays can be greater than variation between arrays • Come out with different results every time

  7. Torture the data long enough and they will confess to anything. • (David G, Merck Co.)

More Related