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seamless cloning

FreeClone™ Seamless Cloning has many advantages compared to conventional restriction enzyme/ligation cloning.<br><br>No restriction digest of the DNA fragments after PCR is necessary. No restriction site scar remains between two DNA fragments.<br>The backbone vector can be digested, or synthesized by PCR.<br>It is far simpler than conventional cloning schemes, as it requires fewer steps. It takes only 20 minutes for assembly reaction and transformation.<br>Multiple DNA fragments can be combined simultaneously in a single-tube reaction.

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seamless cloning

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  1. Seamless cloning

  2. FreeClone™ Seamless Cloning has many advantages compared to conventional restriction enzyme/ligation cloning. • No restriction digest of the DNA fragments after PCR is necessary. No restriction site scar remains between two DNA fragments. • The backbone vector can be digested, or synthesized by PCR. • It is far simpler than conventional cloning schemes, as it requires fewer steps. It takes only 20 minutes for assembly reaction and transformation. • Multiple DNA fragments can be combined simultaneously in a single-tube reaction. • http://www.creative-biogene.com/Product/FreeClone-TM-Seamless-Cloning-kit.html

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