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Investigating Global Water Issues: Introduction to Ion Chromatography

Explore the global water crisis by investigating fluoride levels in water using ion chromatography. Learn about the importance of clean water and the impact of fluoride. Engage in interdisciplinary STEM education through research-based techniques.

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Investigating Global Water Issues: Introduction to Ion Chromatography

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  1. Researching Global Water Issues: Introduction to Ion ChromatographyResearch-Based, Interdisciplinary STEM Education (RISE)

  2. Interdisciplinary Investigations (IDI)-LAB Chemistry 161 Lab Chemistry 163 Lab Chemistry 162 Lab Instrumentation Investigative Thinking 3-5 week research Project Investigative Thinking This Investigation Pre-Lab Part 1: Conceptual Understanding IC Part 2: Wet-Lab - Solution Preparation and Analysis using the IC Part 3: Seminar-Scientific Literature Part 4: Representing Results & IDI Challenge Post-lab Assignment Introduction to instrumentation

  3. Global Water Crisis The availability of clean and plentiful water is fast becoming one the most pressing global challenges, with far-reaching consequences for the health and well-being of all living organisms. What Issues do you know about?

  4. Global Water Crisis – Focus on F- Lets Watch! American Chemical Society: Fluoride & Teeth ScienceNation: Fluorosis in Africa

  5. Part 1: Introduction to Ion Chromatography How do we know if water contains too much Fluoride (F-)? Chromatography: Chromatography is a general term used to describe a collection of laboratory techniques designed to separate mixtures Fun Animation of General Idea

  6. Part 1: Introduction to Ion Chromatography

  7. Part 1: Introduction to Ion Chromatography Groups of 4 researchers needed for this project. Let’s take a Tour of North’s IC!

  8. Part 1: Introduction to Ion Chromatography Complete Part 1 with your Group

  9. Part 1: Introduction to Ion Chromatography Anion Exchange

  10. Part 1: Introduction to Ion Chromatography Cation Peaks 1. lithium (Li+) 2. sodium (Na+) 3. ammonium (NH4+) 4. potassium (K+) 5. unknown 6. unknown 7. magnesium (Mg2+) 8. calcium (Ca2+)

  11. Part 1: Introduction to Ion Chromatography sulfate (SO42-) nitrate (NO3-) fluoride (F-) chloride (Cl-) bromide (Br-) phosphate (PO43-) nitrite (NO2-)

  12. Part 2: Wet-Lab – Solution Preparation & Analysis Using the IC • Take out your Prelab assignment and compare calculations with your group members. • 2. Be sure to use ultrapure water today • 3. Be sure to use proper technique for both the micropipettes and volumetric flask.

  13. Part 3: Seminar Assignment for Next Time: 1. Read the article “Second Thoughts about Fluoride” by Dan Fagin 2. Write a summary of the article 3. Write three questions about the article 4. Bring the summary and questions with you to class and be prepared to participate in an in-class seminar

  14. Part 4: Representing Results sulfate (SO42-) nitrate (NO3-) fluoride (F-) chloride (Cl-) bromide (Br-) phosphate (PO43-) nitrite (NO2-)

  15. Part 4: Representing Results & Challenge

  16. Part 4: Representing Results & Challenge Identify the retention time of fluoride (F-). Are there any other peaks in your chromatograph? If so, discuss what these might be. Make a table including the known concentration of each solution and the given area under each of these peaks. Use Excel to create a calibration curve using your known solution concentrations and the IC results.  

  17. Part 4: Representing Results & Challenge Challenge What is the F- concentration in the two water samples? What did the fluoride ion concentrations you determined tell you about these municipalities? Discuss in your group what you think about the controversy over fluoride as an additive to public drinking water.

  18. Part 4: Representing Results & Challenge Challenge What is the F- concentration in the two water samples? What did the fluoride ion concentrations you determined tell you about these municipalities? Discuss in your group what you think about the controversy over fluoride as an additive to public drinking water.

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