2 nd link: cellular protection

1. Free radicals 2nd link: cellular protection 2nd key player for health = Anti-free radical defences, the HSP

2. Free radicals Cellular aggression and protection: Anti-free radical defence The neutralisers: enzymes and their cofactors:S.O.D: Cu, Zn, Mn G.P.X: Se Catalase: Fe Free radical scavngers Vitamins A, C, E, β-carotene, flavonoides, (diet), etc.

3. 2nd link: cellular protection 2nd key factor for health = Anti-free radical defence system, HSPs UV rays, Inflammation, Infection Alteration of structural or physiological proteins (enzymes, hormones, etc.)

4. It is also… UV rays, Thermic shock, Inflammation, Infection Changes in structural or physiological proteins (enzymes, hormones, etc.) Stress proteins (HSP) Repairing or evacuating proteins(Chaperon)

5. Dietary advice Anti-oxidant model Fresh fruit and vegetables Source of carotenoids Herbs and spices, tea, wine Perturbed cellular protection Advice on supplements Le réflexe Oxybiane Le réflexe Proteochoc

6. Think Proteochoc 2 situations From the very first symptoms of… INFLAMMATION INFECTION  Trauma, bruising  Flu-like symptoms  Joint pain  Lip and genital herpes  Burns, sunburn  Mouth ulcers  Sprains  Tooth abscess • Regimen: 4 capsules a day for 3 days

7. Like certain other algae and plants, the seaweed Porphyra umbilicalis can survive under extremely harsh conditions About Proteochoc Porphyral extract was added to the first Oxybiane composition in 1993 (?), for its anti-oxidant and anti-elastase properties. In 1995, Dr Paul Bringer and Dr Jean-Louis Lichon observed the effects of Oxybiane on dry eyes and cataracts, suggesting that the product, and in particular Porphyral, might have other properties.

8. About Proteochoc = In 1999, PiLeJe became involved in a stress protein modulator development project. = Meeting with HSP specialist Professor André Patrick ARRIGO* (CNRS LYON 1). He suggested that Porphyral had a HSP-modulating effect. = Research projects began P.U. type plants and algae are known to have the ability to synthesise HSP rapidly *Médecines/Sciences n°1, vol 14, janvier 1998

9. Global antioxidant potential of Porphyral HSP® - KRL test- 300 250 200 Increase in anti-free radical resistance (% control blood) 150 100 50 0 0 0,1 0,2 0,5 1 2 5 10 concentration (g/L) Effects of a Porphyral HSP® extract on the DNA damage induced by UV rays 400 200 Light intensity (RLU) as a proportion of number of DNA lesions 0 Control without UV Porphyra 0.1% Porphyra 1.3% The different studies 1- Porphyral HSP* boosts anti-free radical resistance 2- Porphyral HSP* reduces UV ray-induced DNA damage

10. Porphyral HSP Témoin Augmentation des HSP 27, 60, 70 Plus rapide pour 27 et 60 3- Porphyral HSP* stimulates HSP production after thermal shock

11. PiLeJe patents the Porphyra umbilicalis extraction process under the name Porphyral HSP* These studies were presented at the 4th Micronutrition Symposium in March 2002 by Flore MARTIN, head of the scientific department Protocol evaluating the modulatory effects of Porphyra umbilicalis on stress protein synthesis