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Institute for Biomolecular Science (IBS) 14 Academic Staff Dept. of Chemistry Dept. of Biology 9 Postdocs 30 PhD Students B. Med Chem B. Biotech . Project areas include: Drug design and development Biomolecular structure determination Cellular and molecular biology
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Institute for Biomolecular Science (IBS) 14 Academic Staff Dept. of Chemistry Dept. of Biology 9 Postdocs 30 PhD Students B. Med Chem B. Biotech Project areas include: Drug design and development Biomolecular structure determination Cellular and molecular biology Food and natural product chemistry Mechanism of cataract formation Metals in Medicine Research University of Wollongong
How does Chrysotherapy Work? • Many possible mechanisms of action: • Anti-inflammatory • Inhibition of lysosomal enzymes • Immunomodulatory activity • Effects on metabolism of reactive oxygen • All gold drugs rapidly bind to proteins containing thiol side chains, but not DNA. • [Au(CN)2]- is a common metabolite of all Au drugs • Au(I) oxidised to Au(III) in vivo
ESI-MS of Human Serum Albumin (HSA) J. Croft, CHEM340, 2004
Reaction between [Au(PEt3)Cl] and Metallothionein S. Ambehara, B.Sc(Hons), 20002
Covalent Binding of Ruthenium Compounds to DNA J. Torrens, B.Sc(Hons), 2000
Non-covalent Interactions Between Ruthenium Complexes and DNA J. Aldrich-Wright and co-workers
ESI-MS of D2 [D2-6H]6- [D2-5H]5- T. Urathamakul, B.Sc(Hons), 2002
ESI-MS of [Ru(phen)2(dpqC)]2+(mol. wt = 748) T. Urathamakul, B.Sc(Hons), 2002
Reactions between [Ru(phen)3]2+ and D2 T. Urathamakul, B.Sc(Hons), 2002 Ru:D2 = 5:1 Ru:D2 = 10:1 Ru:D2 = 20:1 Ru:D2 = 30:1 Ru:D2 = 50:1
Reactions between [Ru(phen)2L]2+ and D2 L = phen L = pda L = dpq L = dpqMe2 L = dpqC T. Urathamakul B.Sc(Hons), 2002 L = dppz
Future Work • Binding of mono- and poly-nuclear ruthenium complexes to longer DNA and DNA containing other base sequences • Binding of gold, vanadium, bismuth, ruthenium and other metal-containing drugs to proteins including HSA, MT and Transferrin