LOUISIANA Cardiovascular, Renal and Hypertension Centers of Excellence

1. LOUISIANACardiovascular, Renal and Hypertension Centers of Excellence “Mentoring in Cardiovascular Biology” NIH/NCRR CoBREGRANT “Research Excellence in Hypertension and Renal Biology” NIH/NCRR CoBREGRANT P20 RR018766 P20 RR017659 Daniel R. Kapusta, Ph.D.P.I. and Director Professor Department of Pharmacology LSU Health Sciences Center Patrice Delafontaine, M.D. Co-Director Professor of Cardiovascular Medicine Chief, Section of Cardiology Director, Tulane Cardiovascular Center of Excellence Tulane University School of Medicine L. Gabriel Navar, Ph.D.P.I. and Director Professor and Chairman, Department of Physiology Co-Director, Tulane Hypertension & Renal Center of ExcellenceTulane University School of Medicine L. Lee Hamm, M.D. Co-Director Professor and Chairman , Department of Internal Medicine Co-Director, Tulane Hypertension & Renal Center of Excellence Tulane University School of Medicine

2. Cardiovascular Disease in Louisiana • In 2009, 80 million American adults (1 in 3) were estimated to have one or more types of cardiovascular disease, including: • High blood pressure • Coronary heart disease • Heart failure • Stroke • Congenital cardiovascular defects • Cardiovascular disease is the #1 killer in America, with an average of one death every 36 seconds. • The State of Louisiana has the 4th greatest prevalence of cardiovascular disease in the nation. • Cardiovascular disease, including heart disease and stroke, was the #1 killer of Louisianans in 2005, accounting for 31% of all deaths.

3. LSUHSC CoBRE Directors Daniel R. Kapusta, PhD Pharmacology Patrice Delafontaine, MD Cardiology Mentoring in Cardiovascular Biology Division of Research InfrastructureNational Center for Research ResourcesNational Institutes of Health

4. Tulane CoBRE Directors LG Navar, PhD Physiology LL Hamm, MD Nephrology Center of Biomedical Research Excellence in Hypertension and Renal Biology Division of Research InfrastructureNational Center for Research ResourcesNational Institutes of Health

5. CoBRECommon Goals Continue to expand and improve cardiovascular research in Louisiana by: • Enhancing the infrastructure and support of existing and new core facilities • Providing strong mentoring and enhanced research support to junior investigators • Assisting junior investigators in becoming independent investigators • Expanding the critical mass of NIH funded investigators at LSUHSC and THSC • Building effective scientific collaborations between basic and clinician scientists • Enhancing translational research in cardiovascular biology and hypertension, which will make significant impact in improving the quality of health in the State of Louisiana

6. Infrastructure Optimization of CORE Laboratories and Equipment Research Laboratory Renovations

7. Components of LSUHSC CoBRE • Administrative Core • Administrative Coordinator, Lynn Lallier, MBA • Cell and Molecular Analysis CORE • Supports proteomics, cell culture, flow cytometry, HPLC and electron paramagnetic resonance (EPR) technologies. • Director, Emel Songu-Mize, PhD Co-Director, Barry Potter, PhD • Imaging and Histology CORE • Provides microscopy of live-cells and histological specimens treated with various stains and immunohistochemical antibodies. • Director: Becky Worthylake, PhD Co-Director: Dana Troxclair, MD • Vascular and Cardiac Function CORE • Provides service for whole animal models of cardiovascular disease and in vivo assessment of cardiovascular and kidney function. • Director: Kurt Varner, PhD • Junior Investigator Projects • LSUHSC Proteomics Core • Chau-Wen Chou, PhD Co-Director, Jessie Guidry, MS

8. Components of Tulane CoBRE • Administrative Core • Administrative Coordinator: Nina Majid, BA, BS • Molecular, Imaging and Analytical CORE • Improve and maintain analytical, molecular, and imaging facilities • Director: Hiroyuki Kobori, MD, PhD • Transgenic and Gene-Targeted Animal CORE • Supports the breeding colonies of gene targeted mice and rats • Director: Ihor V. Yosypiv, MD • Cardiovascular and renal mouse phenotyping(made possible by P30HL101285) • Supports the phenotyping studies in mice • Director: YumeiFeng, MD, PhD • Junior Investigator Projects

9. LSUHSC C0BRESuccess in past JI’s Acquiring Extramural Support Years 1-5 (2003-2008) Project 3 Andrew Catling, Ph.D. Integration of Growth Factors and Integrin Signaling in VSMC Project 2 Frank Park, Ph.D. Gene Transfer, Oxidant Stress, and Diabetic Vascular Disease Project 1 HamidBoulares, Ph.D. PARP1 and Oxidant Stress in the Vasculature Project 4 Guangyu Wu, Ph.D. ER to GOLGI Trafficking of S?ignaling Proteins in Cardiac Myocytes and VSMC Project 5 Gregory Dick, Ph.D. Vascular smooth muscle plasticity in nitric oxide deficiency Project 6 Cuihua Zhang, Ph.D. Role of Inflammation in Endothelial Reperfusion Injury Project 7 Petra Rocic, Ph.D. Cell-Specific ROS Involvement in the Regulation of Coronary Collateral Growth Project 8 Eric Lazartigues, Ph.D. Brain-Targeted ACE2 Overexpression and Blood Pressure Regulation 5 JIs successfully obtained NIH Extramural Grant Support 1 JI successfully obtained AHA Grant Support

10. LSUHSC C0BRESuccess in past JI’s Acquiring Extramural Support Years 1-6 (2003-2009) Project 3 Andrew Catling, Ph.D. Integration of Growth Factors and Integrin Signaling in VSMC Project 2 Frank Park, Ph.D. Gene Transfer, Oxidant Stress, and Diabetic Vascular Disease Project 1 HamidBoulares, Ph.D. PARP1 and Oxidant Stress in the Vasculature Project 4 Guangyu Wu, Ph.D. ER to GOLGI Trafficking of S?ignaling Proteins in Cardiac Myocytes and VSMC Project 5 Gregory Dick, Ph.D. Vascular smooth muscle plasticity in nitric oxide deficiency Project 6 Cuihua Zhang, Ph.D. Role of Inflammation in Endothelial Reperfusion Injury Project 7 Petra Rocic, Ph.D. Cell-Specific ROS Involvement in the Regulation of Coronary Collateral Growth Project 8 Eric Lazartigues, Ph.D. Brain-Targeted ACE2 Overexpression and Blood Pressure Regulation 6 JIs successfully obtained NIH Extramural Grant Support 1 JI successfully obtained AHA Grant Support

11. CURRENTLSUHSC CoBREJunior Investigators Years 6-7 (2008-present) Regulation of VSMC Function by the Insulin Signaling Pathway Mechanisms Involved in the Intracellular Retention of α2C Adrenergic Receptors • Project 9 • Cooper Woods, Ph.D. • Staff Scientist, • OchsnerClinic Foundation • Insulin resistance on vascular response to injury • Project 10 • CatalinFilipeanu, M.D., Ph.D. • Assistant Research ProfessorPharmacology - LSUHSC • GPCR export trafficikng in VSMC • Molecular chaperones and vascular disease AHA Grant Regulation of endothelial cell permeability by Rho/ROCK signaling GAPDH and its Protective role in atherosclerosis • Project 11 • Jerome Breslin, Ph.D. • Assistant Professor, • Physiology – LSUHSC • Intracellular signaing and microvascular leakage in cardiovascular diease • Project 12 • SergiySukhanov, Ph.D. • Assistant Research Professor, • Cardiology – Tulane HSC • Cellular anti-oxidant roleof GAPDH in VSMC AHA Grant

12. Years 1-5 (2002-2008) TULANE CoBRESUCCESS in past JI’s Acquiring Extramural Support Fruzsina Johnson, MD Heme-HemeOxygenase-Carbon Monoxide System in Salt-Induced Hypertension Albert Dreisbach, MD The Role of Genetic Polymorphisms in the Epoxygenase Pathway in Hypertension Keith E. Jackson, PhD Hemeoxygenase in angiotensin II hypertension Hiroyuki Kobori , MD , PhD Tubular Renin-Angiotensin System in Hypertension Paul Muntner, PhD Beneficial Effects of Physical Activity on Blood Pressure among African-American Females Igor Iosipiv, MD Angiotensin in distal nephronontogeny Kristi Reynolds, PhD, MPH Effects of soybean protein on adipocytokines, systemic inflammation, and endothelial dysfunction Lisa Harrison-Bernard, PhD Angiotensin Receptors in Renal Microvascular Physiology Fady T. Botros, PhD Hemeoxygenase in angiotensin II hypertension 4 JIs successfully obtained NIH Extramural Grant Support 5 JIs successfully obtained OTHER Grant Support

13. CURRENTTULANE CoBREJunior Investigators Years 6-7 (2008-present) Transcriptional Control of Ureteric Bud Growth and Branching Citrate transport in the proximal tubule • Project 1 • ZubaidaSaifudeen, PhD, • Assistant Professor, • Department of Pediatrics, Section of Nephrology, • Tulane School of Medicine • role of p53 protein in early kidney development and congenital kidney defects • Project 2 • Kathleen S. Hering-Smith, PhD, • Research Assistant Professor,Department of Medicine, • Tulane School of Medicine • - • acute regulation of citrate transport by calcium, and chronic regulation of citrate transport by acid-base. Tulane Research Enhancement Fund VA Grant Distal Nephron Renin and Prorenin Receptor in Angiotensin II-Dependant Hypertension Macronutrient Composition of Diet & Risk Factors for Cardiovascular Disease (CVD) • Project 3 • Minolfa C. Prieto, MD, PhD, • Assistant Professor, • Department of Physiology, • Tulane School of Medicine • Gene expression and regulation of intrarenal RAS in hypertension. • Regulation of renin in collecting ducts. • Project 4 • Lydia A. Bazzano, MD, PhD, MPH, • Assistant Professor of Epidemiology, Department of Medicine, • Tulane School of • Public Health & Tropical Medicine • long-term effects of a diet low in carbohydrates on CVD risk factors in obese adults. AHA Grant NIH K-Award

14. CURRENTTULANE CoBREJunior Investigators Years 6-7 (2008-present) Mechanism of Resistance Artery Structural Remodeling in Hypertension Endothelial Dysfunction, Adipocytokins, Inflammation & Chronic Kidney Disease (CKD) • Project 5 • Jing Chen, MD, • Assistant Professor of Epidemiology,Department of Medicine, Tulane School of Public Health& Tropical Medicine • effects of endothelial dysfunction, adipocytokines, and inflammation on the risk of CKD. • Project 6 • Khalid Matrougui, PhD, • Assistant Professor, • Department of Physiology, • Tulane School of Medicine. • role of Oxidative Stress, NFkB, av-b3-integrin, and TGFb1 in the remodeling of resistance arteries in hypertension. Just Awarded! NIH R01 Identifying the Role of Microvascular Structure in Hypertension Intrarenal Angiotensin II generation during Angiotensin II-induced hypertension • Pilot Project 7 • Walter Lee Murfee, PhD • Assistant Professor of • Biomedical Engineering, • Department of Biomedical Engineering, • Tulane University • mechanisms involved with microvascular rarefaction associated with Hypertension and how they influence local hemodynamics. • Pilot Project 8 • Romer A. Gonzalez, MD, PhD, • Assistant Professor, • Department of Physiology, • Tulane School of Medicine • mechanisms of angiotensin II synthesis in the kidneys and its role in the development of hypertension. LA Board of Regent Award NIH K-Award

15. TULANE CoBRE External Advisory Committee (EAC) members EAC members (left to right), Dr. Coffman, Dr. Moe, Dr. Rosenblum, and Dr. McGiff Missing from the group picture is Dr. Klag

16. LSUHSC CoBREJr. Faculty Investigator Success Stories • HamidBoulares, Ph.D. • Associate Professor • Department of Pharmacology,LSUHSC-NO, Louisiana • Andy Catling, Ph.D. • Associate Professor, • Department of Pharmacology LSUHSC-NO, Louisiana • Guangyu Wu, Ph.D. • Associate ProfessorDepartment of Pharmacology LSUHSC-NO, Louisiana • Eric Lazartigues, Ph.D. • Assistant Professor • Department of Pharmacology, LSUHSC-NO, Louisiana • (currently being considered for promotion to Associate Professor) • YumeiFeng, Ph.D. • Assistant Professor • Department of Physiology,Tulane HSC-NO, Louisiana • Frank Park, Ph.D. • Associate Professor • Departments of Medicine and Physiology, • Medical College of Wisconsin, Milwaukee, WI • Petra Rocic, Ph.D. • Assistant Professor of Biochemistry and Molecular Biology, • University of South Alabama College of Medicine, Mobile, AL • Cuihua Zhang, Ph.D. • Associate Professor of Internal Medicine, • Medical Pharmacology and Physiology at theUniversity of Missouri, Columbia, MO • Gregory Dick, Ph.D. • Assistant Professor, • West Virginia University’s Robert C. Bird HSC, Morgantown, WV • Richard Wainford, Ph.D. • Instructor • Department of Pharmacology, LSUHSC-NO, Louisiana

17. Tulane CoBREJr. Faculty Investigator Success Stories Albert W. Dreisbach, MD Chair of the ASCPT Nephrology and Hypertension Scientific Section, University of Mississippi Medical Center, Jackson, MS. Lisa M. Harrison-Bernard, PhD Associate Professor of Physiology, Louisiana State University Health Sciences Center, LA Fruzsina Johnson, MD Assistant Professor, University of Texas, San Antonio, TX. Ihor V. Yosypiv, MD Associate Professor of Pediatric Medicine Director, THRCE Transgenic & Gene-Targeted Animal Core Tulane Medical School, LA. Keith E. Jackson, PhD Asst. Professor of Pharmacology University of Louisiana at Monroe, LA Hiroyuki Kobori, MD, PhD Associate Professor, Department of Physiology; Director, THRCE Molecular & Imaging Core Facility Tulane Medical School, LA. Kristi Reynolds, PhD, MPH Outcome Investigator Kaiser Permanente, Los Angeles, CA Paul Muntner, PhD Professor of Epidemiology & Medicine, Department ofMedicine, Section of Epidemiology University of Alabama at Birmingham, Birmingham, AL. Fady T. Botros, PhD Senior Scientific Communications Associate, Global Medical Communications Eli Lilly and Company.

18. LSUHSC Translational Research in Cardiovascular Biology Inflammatory Mediators Lead to Carotid Plaque Instability and Rupture Pilot Project 1 HernanBazan, M.D. Assistant Professor Surgery LSUHSC Diminished omega-3 fatty acids are associated with carotid plaques from neurologically symptomatic patients; Implications for carotid intervention. Improved Health Care in Louisiana Surgery LSUHSC Pharmacology Nephrology Planned Clinical Trial (PI: DR Kapusta) Novel therapeutic approach to maintain renal function, avoid renal replacement therapy and improve outcome in intensive care unit (ICU) patients with trauma and hemorrhage

19. Tulane Translational Research in Kidney Biology and Hypertension

20. CoBRE Grant Support “Mentoring in Cardiovascular Biology” P20 RR018766 “Center of Biomedical Research Excellence in Hypertension and Renal Biology” P20 RR017659 Division of Research Infrastructure National Center for Research Resources National Institutes of Health