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EDI. EDII. IIICS. EDI. NH2. COOH. Minigene. 20 splicing isoforms. PAGE. pre-mRNA. EDI. EDI +. RT-PCR. EDI. EDI -. EDI - mRNA. EDI + mRNA. Mu ñoz et al. Figure 1. a. b. Fibronectin. d. c. Mu ñoz et al. Figure 2. 7 tet operators. EDI. a. P hCMV*-1. pUHC-EDA.
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EDI EDII IIICS EDI NH2 COOH Minigene 20 splicing isoforms PAGE pre-mRNA EDI EDI+ RT-PCR EDI EDI- EDI- mRNA EDI+ mRNA Muñoz et al. Figure 1 a b Fibronectin d c
Muñoz et al. Figure 2 7 tet operators EDI a PhCMV*-1 pUHC-EDA VP16 acidic domain tetR PCMV PCMV tetR Sp1 Gln rich b tTA-Sp1 tTA-VP16 aa 1207 aa 268499 aa 1207 aa 363490
EDI Muñoz et al. Figure 3 -amanitin resistant pol II (large pol II subunit) reporter minigene Asn 792 Asp WT o mutant EDI endogenous pol II RNAPII -amR -amanitin (24 hr) Hep3B (human hepatoma) Endogenous pol II -am