Technical BioImaging

1. Technical BioImaging TIF045 5p 24:th October-9:th December To see is to understand... http://www.fy.chalmers.se/atom/research/cars/courses Contact: Annika Enejder, enejder@fy.chalmers.se

2. Aims Technical BioImaging To understand how a 3-dimensionell, highly resolved image is created in a modern light microscope The physics behind light-matter interaction in a microscopic probe volume How cell structures and even biomolecules can be imaged and manipulated in a microscope Give a flavor of all the novel microscopy techniques currently emerging within the exciting inter-disciplinary field of physics and molecular cell biology Contact: Annika Enejder, enejder@fy.chalmers.se

3. Technical BioImaging High-lights from the program: The Nikon Microscopy Workshop at Chalmers University 26-27:th October From the fundamentals on the anatomy of a modern microscope to hands-on training in how to use a microscope & collect images of biological samples Contact: Annika Enejder, enejder@fy.chalmers.se

4. Project conducted on the research microscopes at The Centre for BioPhysical Imaging14-18:th November Technical BioImaging High-lights from the program: • Confocal microscopy of GFP labeled proteins • 2-photon fluorescence microscopy • Fluorescence Lifetime Imaging – FLIM • CARS - Coherent Anti-Stokes Raman Scattering microscopy • Vibrational (Raman) microscopy • Surface Plasmon Resonance and Nanoparticle sensors • Optical tweezers and manipulation

5. Technical BioImaging High-lights from the program: Lecture series by Prof James B Pawley University of Wisconsin, Madison, USA 21-23:th November The editor of the course literature: Handbook of Biological Confocal Microscopy

6. Technical BioImaging Course Contents: The light microscope has traditionally been our most important instrument to catch a glimpse of the fascinating micro-cosmos of biological cells. The fundamental optics of the microscope and its function – to image with an ever increasing magnification and resolution – has been well-known for several hundred years. However, the information provided by the conventional “snapshots” of transmitted light is no longer enough within the modern biosciences in order to gain full understanding of the complex processes life comprises. The requirements on resolution and contrast in order to visualize the “invisible” have the past decade drastically been changed; we now want to be able to monitor biochemical processes in living cells on molecular level and with high temporal resolution rather than merely visualizing cellular structures. This has motivated physicists in close collaboration with biologists to develop a range of sophisticated microscopy methods, where the conventional light source is replaced by technically advanced laser systems. Micro-spectroscopic analysis and imaging of biomolecules and molecular markers in the sample is done by inducing fluorescence and vibrational processes. Living cells are captured in the probe volume and manipulated with laser tweezers. The light emitted from the sample, sometimes only single photons, is wavelength-selectively filtered and projected onto a sensitive detector. An image is formed and finally analyzed by means of powerful image analysis software. These advanced laser-based imaging systems set high and new requirements on the user of modern microscopes, one of which is good knowledge in physics. Here we intend to bring up concepts from a wide range of fundamental physics courses and use them to understand how a 3-dimensionell picture is created in a modern light microscope, how cell structures and biomolecules selectively can be imaged and manipulated in fluorescence and spectroscopically based microscopes, and how important image analysis and sample preparation is for the final results. Contact: Annika Enejder, enejder@fy.chalmers.se

7. Technical BioImaging TIF045 5p For further information, contact Annika Enejder enejder@fy.chalmers.se http://www.fy.chalmers.se/atom/research/cars/courses