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Nucleotide Structure: Pentose sugar

Nucleotide Structure: Pentose sugar. Nucleotide Structure: Nitrogenous Bases. DNA Linkage. Phosphate group/phosphodiester bond Nucleotides contain one, two, or three phosphate groups. *nucleoside *nucleotide. Nucleic Acid Polarity. *Top of molecule has free 5’-phosphate group

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Nucleotide Structure: Pentose sugar

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  1. Nucleotide Structure: Pentose sugar

  2. Nucleotide Structure: Nitrogenous Bases

  3. DNA Linkage • Phosphate group/phosphodiester bond • Nucleotides contain one, two, or three phosphate groups *nucleoside *nucleotide

  4. Nucleic Acid Polarity *Top of molecule has free 5’-phosphate group This is the 5’ end *Bottom has a free 3’ hydroxyl group This is the 3’ end

  5. Structural analysis of DNA *Early 1950’s Rosalind Franklin *1953 Watson and Crick

  6. Complementarity

  7. DNA Structure:

  8. Brief Review of DNA Replication:

  9. Nearest Neighbor Analysis (Kornberg 1956): In vitro DNA synthesis •Template DNA •DNA polymerase •dNTPs *dATP *dCTP *dGTP *dTTP •Allow DNA replication to take place •Denature DNA •Treat with micrococcal nuclease- yields mononucleotides with 3’ terminal phosphates

  10. ssDNA treated with micrococcal nuclease: A A C C C * G G T T T T C ssDNA: *

  11. Exercise: List all possible dinucleotides (analysis of nearest 5’ neighbor) Which must be present in equal frequencies if the DNA strands are: Parallel ?Antiparallel?

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