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Effect of the Gonadopeptide Inhibin on Fundulus heteroclitus Oocyte Maturation

Effect of the Gonadopeptide Inhibin on Fundulus heteroclitus Oocyte Maturation. Y-W Peter Lin, Tericka M Smith, and Teresa R Petrino School of Natural and Health Sciences, Barry University, Miami Shores, Florida. Pituitary. Gonadotropins. LH FSH. Steroids. Granulosa cell. T, E, P.

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Effect of the Gonadopeptide Inhibin on Fundulus heteroclitus Oocyte Maturation

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  1. Effect of the Gonadopeptide Inhibin on Fundulus heteroclitus Oocyte Maturation Y-W Peter Lin, Tericka M Smith, and Teresa R Petrino School of Natural and Health Sciences, Barry University, Miami Shores, Florida

  2. Pituitary Gonadotropins LH FSH Steroids Granulosa cell T, E, P Gonadal peptides Inhibin Activin Fallistatin Oocyte

  3. Pituitary-Ovarian Axis • Endocrine (Long distance) • Paracrine (Local, acts on adjacent cell) • Autocrine (acts on the same cell)

  4. Pituitary Gonadotropins LH FSH Steroids Granulosa cell T, E, P Gonadal peptides Inhibin Activin Fallistatin Oocyte

  5. Fundulus heteroclitus • Permission to use the above image granted by Sportfishing and Aquatic Resources Education Program (SAREP) • http://www.sarep.cornell.edu/Sarep/index.htm

  6. 40 x Fundulus ovary 60 x Germinal vesicle follicle

  7. Inhibin effects on Fundulus Oocyte Maturation induced by steroid • Germinal Vesicle Breakdown (GVBD) • 17alpha,20beta-dihydro-4-pregnene-3-one (DHP)

  8. Pituitary Gonadotropins LH FSH Steroids Granulosa cell T, E, P Gonadal peptides Inhibin Activin Fallistatin Oocyte

  9. Inhibin effects on Fundulus Oocyte Maturation induced by Gonadotropin • Fundulus Pituitary Extract (FPE) • Pituitary Equivalent per ml (PE/ml)

  10. Inhibin effects on Fundulus Ovarian Follicle Steroid Production • DHP Steroid Radioimmunoassay (RIA)

  11. Pituitary Gonadotropins LH FSH Steroids Granulosa cell T, E, P Gonadal peptides Inhibin Activin Fallistatin Oocyte

  12. Inhibin effects on Denuded Oocyte • Denuded Oocyte: oocyte without the surrounding granulosa cells, theca and surface epithelium

  13. Inhibin effects on Ovarian Follicle Atresia • Atretic Follicle: follicle that undergoes degenerative change, damage follicle

  14. Conclusions • Inhibin effectively blocked or delayed the progression of meiosis (GVBD) initiated by both steroid (DHP) and gonadotropin (FPE) in a dose-dependent manner. • The inhibitory action of inhibin was reversible. With longer incubation time, higher doses of inducers overcame the inhibitory effect of inhibin.

  15. Steroidogenesis (DHP production induced by gonadotropin) was not significantly blocked in the presence of inhibin. • Both spontaneous maturation and GVBD induced by steroid in denuded oocytes were inhibited by inhibin.

  16. Inhibin may act directly on the oocyte to block the resumption of meiosis (GVBD). • Results from this study indicate that inhibin may act as a paracrine modulator of oocyte maturation because its effect was dose dependent, not permanent or deleterious.

  17. Acknowledgements • Research supported by NIH-MBRS SCORE Grant GM45455-08 • NIH-MARC U*STAR Grant GM08021-17 • Barry University Scholarship Grant • FASEB Travel Grant

  18. Pituitary Gonadotropins LH FSH Steroids Granulosa cell T, E, P Gonadal peptides Inhibin Activin Fallistatin Oocyte

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