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Drawing blood samples

Drawing blood samples . EHES Training Materials. Exclusion criteria. Blood samples are not taken, if participant Has a chronic illness which restricts taking blood samples Has anemia ( Hb below 10 g/l) Doubts about the blood volume to be taken Refuses. Equipment .

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Drawing blood samples

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  1. Drawing blood samples EHES Training Materials

  2. Exclusion criteria Blood samples are not taken, if participant • Has a chronic illness which restricts taking blood samples • Has anemia (Hb below 10 g/l) • Doubts about the blood volume to be taken • Refuses

  3. Equipment • Blood sampling vacuum tubes • Needle for vacuum tubes • Needleholder/adapter • Needle disposal container • Stasis • Disinfection solution, swabs, gauze pads, skin tape • Disposable gloves

  4. Blood collection tubes • Serum evacuated tubes containing gel or plain serum tubes (red cap) • Fluoride-citrate plasma tube (grey cap) • EDTA plasma tubes (violet cap)

  5. Preparing for the blood collection • Check participant’s ID code • Place the code label on the form • Record length of time from the last meal in full hours • Participant rests 10-15 minutes • Use gloves or disinfect hands

  6. Posture of the subject • Sitting position, hand downward, palm upwards • Before blood collection, ask the participant to remove tight clothes that might constrict the upper arm • If participant tells that he/she easily faints, supine posture due to safety of the participant • Take blood from the arm not used for BP measurement • usually left arm

  7. Protocol 1/8 • Gently tap top of the fluoride citrate plasma tube if it contains granules • Release cover from the end of the needle and connect the holder and sample needle • Apply tourniquet (if needed) to search a vein • Limit the use of the tourniquet to less than one minute at a time

  8. Protocol 2/8 • Place the arm in a downward position and disinfect the puncture site • Remove the protector case from the needle • Perform the venipuncture

  9. Protocol 3/8 • Collect blood first into the 2 plain serum tubes • Press the tube to puncture the stopper and start filling • Loosen the tourniquet when the bloodstream runs • Support the tube during filling

  10. Protocol 4/8 • When the bloodstream stops, take the tube out of the holder • Check that the tube is filled to the accurate volume • Mix the plain serum tube 5 times by inverting the tube completely top-down immediately after taking the tube out of the holder

  11. Protocol 5/8 • Fill the fluoride-citrate plasma tube • Immediately after taking the fluoride-citrate tube out of the holder, mix 15 times by inverting the tube completely top-down

  12. Protocol 6/8 • Fill the 3 EDTA tubes • Mix the plain EDTA tubes 5 times by inverting the tube completely top-down immediately after taking the tube out of the holder • Store all tubes in a vertical position until sample handling

  13. Protocol 7/8 • Remove the needle from the vein • Apply pressure immediately on the puncture site with a gauze pad until bleeding stops

  14. Protocol 8/8 • Label the successfully filled blood sampling tubes according to the sampling chart • Discard needles into a needle dispose container and all other materials contaminated with blood into a biological waste container

  15. Recording Record: • Deviations from the blood drawing protocol • Your initials/personnel code to identify who draw the blood samples

  16. Acknowledgements • Slides • Laura Lund, Päivikki Koponen • Photographs • Hanna Tolonen • Measurement demonstration • Saara Vallivaara, Maarit Kajosaari

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