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Investigating the effects of Plant Hormones on the growth of A.Niger

Investigating the effects of Plant Hormones on the growth of A.Niger. PRESERVATIVE?. Objectives. Investigate the ability of different plant hormones (or a mixture) to defend tomatoes from fungus Aspergillus niger Way to increase the storage time of tomatoes. Why A.niger ?

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Investigating the effects of Plant Hormones on the growth of A.Niger

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  1. Investigating the effects of Plant Hormones on the growth of A.Niger

  2. PRESERVATIVE?

  3. Objectives • Investigate the ability of different plant hormones (or a mixture) to defend tomatoes from fungus Aspergillusniger • Way to increase the storage time of tomatoes • Why A.niger? • Inflicts tremendous damage to plants and crops • Infects dead, dying or injured plant tissues

  4. Introduction • Known as Black Mould • Affects certain fruits and vegetables • Inflicts damage on dead and injured plant tissues

  5. Introduction • Organic substances • Causes physiological responses

  6. Target audience • Farmers who grow tomatoes • People who want to keep their tomatoes for a longer time • People who want to find out how to reduce mould growth on tomatoes

  7. Hypothesis The plant hormone that best impedes the growth of Aspergillus Niger is salicylic acid.

  8. Materials Aspergillusniger Potato dextrose agar Methyl Jasmonate Potato dextrose broth Salicylic acid Tomatoes Kinetin Syringe Electronic Balance Petri dishes

  9. Methodology

  10. Methodology

  11. We injected 0.5 ml of the Aspergillusniger solution (mixed in potato dextrose broth solution into the tomatoes). We measured the mass of the tomatoes during the following intervals: Start of experiment 2 hours later 1 day later 2 days later Methodology

  12. Methodology Salicylic acid Methyl Jasmonate Kinetin Salicylic acid + Methyl Jasmonate Salicylic acid + Kinetin Methyl Jasmonate + Kinetin All three hormones Control

  13. Factor used to measure the extent of decay • Mass of the tomatoes

  14. Overview of results

  15. Mass of tomatoes (set-up 1)

  16. Mass of tomatoes (set-up 2)

  17. Mass of tomatoes (set-up 3)

  18. Percentage decrease in mass of tomatoes (set-up 1)

  19. Percentage decrease in mass of tomatoes (set-up 2)

  20. Percentage decrease in mass of tomatoes (set-up 3)

  21. Average percentage decrease in mass

  22. Raw Data

  23. General analysis of results • The mass of the tomatoes which were injected with salicylic acid decreased the least • Among the three hormones, salicylic acid works best at impeding growth of Aspergillus Niger, followed by kinetin, and then Methyl Jasmonate

  24. General analysis of results Impedes growth of Aspergillus Niger the most Impedes growth of Aspergillus Niger the least

  25. Explanation of results – salicylic acid

  26. Limitations • We originally intended to use jasmonate acid instead of kinetin. However, jasmonate acid is too expensive and the SRC cannot afford it. Hence, we found a replaced jasmonate acid with kinetin • Using plant hormones as a form of “preservative” for the tomatoes would cost a lot as the hormones are expensive

  27. Conclusion • Salicylic acid works best on impeding growth of Aspergillus Niger among the three plant hormones used in the experiment

  28. Benefits • Injecting plant hormones is more environmentally friendly than using fungicides. Unlike fungicides, plant hormones are not toxic to humans and wildlife. • The fruits injected with this hormones may not be suitable for consumption, and hence this method may not be applicable for agricultural purposes.

  29. Applications • Salicylic acid can be injected into fruits like tomatoes to reduce the Aspergillus Niger growth on the fruits. This increases the storage time of fruits.

  30. Bibliography • http://www.crscientific.com/article-aspirin.htmlFrom CR Scientific LLC (date not applicable) • http://onlinelibrary.wiley.com/doi/10.1002/pca.1085/abstract • Written Feng Juan Zhang, You Ju Jin, Xing You Xu, Rong Chun Lu, • Hua Jun Chen (10 JUL 2008) • http://www.cliffsnotes.com/study_guide/Types-of-Plant-Hormones.topicArticleId-23791,articleId-23713.html#ixzz1AAQ3pfpI • From Cliffnotes.com (4 Apr 2011) • http://www.brighthub.com/health/conditions-treatments/articles/9543.aspx • Written by user  MandaSpring (1 Oct 2008) • http://www.gardenguides.com/132402-aspergillus-niger-structure.html • Written by Sarah Terry (date not applicable)

  31. Bibliography • http://www.biog1105-1106.org/demos/105/unit5/media/plantdefensehormones.pdf • http://www.chm.bris.ac.uk/motm/jasmine/jasminev.htm • http://www.sureshrattan.com/index.php?option=com_content&view=article&id=48&Itemid=68 • http://www.cliffsnotes.com/study_guide/Types-of-Plant-Hormones.topicArticleId-23791,articleId-23713.html#ixzz1AAQ3pfpI • http://www.apsnet.org/edcenter/intropp/topics/Pages/OverviewOfPlantDiseases.aspx • Google Images • Presentation magazine

  32. Thank You

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