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The Effects of Ampicillin on the Growth of E. Coli

The Effects of Ampicillin on the Growth of E. Coli. Michelle Anthony Khadija Lawrence . Escherichia coli . Gram-negative Rod shaped Pathogenic strains . http://www.universityofcalifornia.edu/everyday/agriculture/images/e_coli.jpg. Ampicillin .

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The Effects of Ampicillin on the Growth of E. Coli

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  1. The Effects of Ampicillin on the Growth of E. Coli Michelle Anthony Khadija Lawrence

  2. Escherichiacoli • Gram-negative • Rod shaped • Pathogenic strains http://www.universityofcalifornia.edu/everyday/agriculture/images/e_coli.jpg

  3. Ampicillin http://upload.wikimedia.org/wikipedia/commons/4/48/Ampicillin-2D-skeletal.png

  4. Hypothesis • The maximum growth of E. coli will be inhibited as the concentration of ampicillin is increased.

  5. Components • Culture (E. coli) • Ampicillin • 10x lower LB • H2O

  6. Experiments 1-4 • P. fluorescens • resistant • Titrations • No derived conclusion • Lead to redesign

  7. Experiments 1-4 • P. fluorescens • resistant • Titrations • No derived conclusion • Lead to redesign

  8. Experiments 1-4 • P. fluorescens • resistant • Titrations • No derived conclusion • Lead to redesign

  9. Experiments 5-10 • No titrations • Altered amounts of ampicillin • Varied the ranges each week

  10. Experiments 5-10 • No titrations • Altered amounts of ampicillin • Varied the ranges each week • Results from each week lead to final experiment

  11. Experimental Design * All values in table are in microL

  12. 3 0 5 9 12 16

  13. Conclusion derived from Results • Maximum growth was not inhibited • Normal curve max: 1.088 • Highest amp max: 1.044 • Lag phase dramatically affected after 16microL is exceeded • IC50 not determined • Ampicillin affects length of lag phase • Graph consistent with scale

  14. References • http://www.cdc.gov/ecoli/general/ • http://www.foodsafety.gov/poisoning/causes/bacteriaviruses/ecoli/ • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1583696/?page=4

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