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EPITOPE TAGGING OF RIBOSOMAL PROTEINS. Sst I. Xba I. Nco I. Bam HI. Eco RI. Hind III. ATG. STOP. RB. nptII. CaMV 35S. CaMV 35S. Ω. HF. r-protein. OCS. LB. p35S:HF-RP. Bam HI. Xba I. Sst I. Nco I. Eco RI. Hind III. ATG. STOP. RB. nptII. CaMV 35S. CaMV 35S. Ω.
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SstI XbaI NcoI BamHI EcoRI HindIII ATG STOP RB nptII CaMV 35S CaMV 35S Ω HF r-protein OCS LB p35S:HF-RP BamHI XbaI SstI NcoI EcoRI HindIII ATG STOP RB nptII CaMV 35S CaMV 35S Ω r-protein FH OCS LB p35S:RP-FH A)N-terminus tagging B)C-terminus tagging